Universidade Estadual Paulista "Júlio de Mesquita Filho" - Unesp, School of Dentistry, Department of Dental Materials and Prosthodontics, Araraquara, SP, Brazil.
Universidade Estadual Paulista "Júlio de Mesquita Filho" - Unesp, School of Dentistry, Department of Physiology and Pathology, São Paulo State University (Unesp), School of Dentistry, Araraquara, SP, Brazil.
Braz Oral Res. 2017 Dec 18;31:e104. doi: 10.1590/1807-3107BOR-2017.vol31.0104.
The aim of this study was to evaluate the bioactivity and cytocompatibility of simvastatin (SV) applied to MDPC-23 odontoblast-like cells. For this purpose, MDPC-23 cells were seeded in 96-well plates and submitted to treatments with 0.01 or 0.1 μM of SV for 24 h, 72 h or continuously throughout the experimental protocol. The negative control group (NC) was maintained in DMEM. Cell viability (MTT), ALP activity (thymolphthalein monophosphate), and mineralized matrix deposition (alizarin red) were analyzed at several time points. The data were submitted to ANOVA and Tukey's test (α = 0.05). Although cell viability was observed in the groups treated with SV, these groups did not differ from the NC up to 7 days. There was a reduction in cell viability for the groups treated with 0.1 μM of SV for 72 h, and submitted to continuous mode after 14 days. A significant increase in ALP activity occurred in the group treated with 0.01 μM of SV for 24 h, compared with the NC; however, only the group treated with 0.1 μM of SV in continuous mode reduced the ALP activity, in comparison with the NC. After 14 days, only continuous treatment with 0.1 μM of SV did not differ from NC, whereas the other experimental groups showed increased mineralized matrix deposition. Thus, it was concluded that low concentrations of simvastatin were bioactive and cytocompatible when applied for short periods to cultured MDPC-23 odontoblast-like cells.
本研究旨在评估辛伐他汀（SV）对 MDPC-23 成牙本质样细胞的生物活性和细胞相容性。为此，将 MDPC-23 细胞接种于 96 孔板中，并分别用 0.01 或 0.1 μM 的 SV 处理 24 h、72 h 或整个实验过程中连续处理。阴性对照组（NC）用 DMEM 维持。在不同时间点分析细胞活力（MTT）、碱性磷酸酶活性（百里酚酞单磷酸）和矿化基质沉积（茜素红）。数据进行方差分析和 Tukey 检验（α=0.05）。尽管在 SV 处理组中观察到细胞活力，但在 7 天内这些组与 NC 没有差异。用 0.1 μM 的 SV 处理 72 h 后，细胞活力降低，14 天后连续处理。与 NC 相比，用 0.01 μM 的 SV 处理 24 h 的组中 ALP 活性显著增加；然而，只有连续用 0.1 μM 的 SV 处理的组降低了 ALP 活性，与 NC 相比。14 天后，只有连续用 0.1 μM 的 SV 处理与 NC 无差异，而其他实验组显示矿化基质沉积增加。因此，结论是，在培养的 MDPC-23 成牙本质样细胞中，低浓度的辛伐他汀在短时间内应用时具有生物活性和细胞相容性。
