Department of Dental Materials and Prosthodontics, Araraquara School of Dentistry, São Paulo State University (Unesp), Rua Humaitá, 1680, Araraquara, SP, 14801-903, Brazil.
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Rua Alameda Dr. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil.
Clin Oral Investig. 2019 Sep;23(9):3457-3469. doi: 10.1007/s00784-018-2757-7. Epub 2018 Dec 14.
To assess the biological, antimicrobial, and mechanical effects of the treatment of deep dentin with simvastatin (SV) before application of a glass-ionomer cement (GIC).
Dentin discs were adapted to artificial pulp chambers and SV (2.5 or 1.0 mg/mL) was applied to the occlusal surface, either previously conditioned or not with EDTA (±EDTA). The extracts (culture medium + SV that diffused through dentin) was obtained and then applied to cultured odontoblast-like MDPC-23 cells. Cell viability, alkaline phosphatase (ALP) activity, and mineralization nodule (MN) deposition were evaluated. Untreated discs were used as control. The antibacterial activity of SV (2.5 or 1.0 mg/mL) against Streptococcus mutans and Lactobacillus acidophilus, as well as the bond strength of GIC to dentin in the presence of SV 2.5 mg/mL (±EDTA) were also assessed. The data were analyzed by ANOVA/Tukey tests (α = 5%).
EDTA + SV 2.5 mg/mL significantly enhanced the ALP activity and MN deposition in comparison with the control, without changing in the cell viability (p < 0.05). The association EDTA + SV 2.5 mg/mL + GIC determined the highest ALP and MN values (p < 0.05). SV presented intense antimicrobial activity, and the EDTA dentin conditioning followed by SV application increased bond strength values compared with SV treatment alone (p < 0.05).
SV presents antimicrobial activity and diffuses across conditioned dentin to biostimulate odontoblast-like pulp cells.
The use of SV as adjuvant agent for indirect pulp capping may biostimulate pulp cells thus preserving vitality and function of the pulp-dentin complex.
评估辛伐他汀（SV）处理深牙本质后再应用玻璃离子水门汀（GIC）前的生物学、抗菌和机械性能。
将牙本质片适应于人工牙髓腔，并将 SV（2.5 或 1.0mg/ml）应用于咬合面，之前用 EDTA 处理或不处理（±EDTA）。提取液（扩散通过牙本质的培养基+SV）获得，然后应用于培养的成牙本质细胞样 MDPC-23 细胞。评估细胞活力、碱性磷酸酶（ALP）活性和矿化结节（MN）沉积。未处理的牙本质片用作对照。还评估了 SV（2.5 或 1.0mg/ml）对变形链球菌和嗜酸乳杆菌的抗菌活性，以及 SV 2.5mg/ml（±EDTA）存在时 GIC 与牙本质的结合强度。通过方差分析/Tukey 检验（α = 5%）对数据进行分析。
与对照组相比，EDTA + SV 2.5mg/ml 显著提高了 ALP 活性和 MN 沉积，而细胞活力没有变化（p < 0.05）。EDTA + SV 2.5mg/ml + GIC 的联合作用确定了最高的 ALP 和 MN 值（p < 0.05）。SV 表现出强烈的抗菌活性，与单独使用 SV 处理相比，EDTA 牙本质预处理后再应用 SV 提高了结合强度值（p < 0.05）。
SV 具有抗菌活性，并扩散到经过处理的牙本质中以生物刺激成牙本质细胞样牙髓细胞。
将 SV 用作间接盖髓剂的辅助剂可能会刺激牙髓细胞，从而保持牙髓-牙本质复合体的活力和功能。
