韶子（墨西哥红毛丹）果壳提取物的多酚含量、体外抗氧化活性及化学成分
Polyphenolic content, in vitro antioxidant activity and chemical composition of extract from Nephelium lappaceum L. (Mexican rambutan) husk.
作者信息
Hernández Cristian, Ascacio-Valdés Juan, De la Garza Heliodoro, Wong-Paz Jorge, Aguilar Cristóbal Noé, Martínez-Ávila Guillermo Cristian, Castro-López Cecilia, Aguilera-Carbó Antonio
机构信息
Department of Animal Nutrition, Universidad Autónoma Agraria Antonio Narro, Col. Buenavista, Calzada Antonio Narro No. 1923, Saltillo, 25315, Coahuila, Mexico.
Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Blvd. Venustiano Carranza, Col. República, Saltillo, 25280, Coahuila, Mexico.
出版信息
Asian Pac J Trop Med. 2017 Dec;10(12):1201-1205. doi: 10.1016/j.apjtm.2017.10.030. Epub 2017 Nov 7.
OBJECTIVE
To determinate the recovery of total polyphenolic compounds content, in vitro antioxidant activity and HPLC/ESI/MS characterization of extract from Nephelium lappaceum L. (Mexican rambutan).
METHODS
The rambutan husk extract was obtained by aqueous extraction and a polyphenolic fraction was recovered using Amberlite XAD-16. The total polyphenolic compounds content was determined by the Folin Ciocalteu and butanol-HCI methods. In vitro antioxidant activity was performed using ABTS and ferric reducing antioxidant power methods.
RESULTS
Mexican rambutan husk showed a total polyphenolic content of 582 mg/g and an evident antioxidant activity by ABTS and ferric reducing antioxidant power analysis. The HPLC/ESI/MS assay allowed the identification of 13 compounds, most of which belong to ellagitannins. Geraniin, corilagin and ellagic acid were present in the sample; the mineral composition was also evaluated.
CONCLUSIONS
Rambutan husk cultivated in Mexico is a promising source for the recovery of added value bioactive compounds with antioxidant activity, which have potential applications as bioactive antioxidant agents for the treatment of diseases.
目的
测定红毛丹（墨西哥红毛丹）提取物中总多酚化合物含量的回收率、体外抗氧化活性及进行高效液相色谱/电喷雾电离/质谱表征。
方法
通过水提取获得红毛丹果壳提取物，并用Amberlite XAD - 16回收多酚级分。采用福林 - 酚法和丁醇 - 盐酸法测定总多酚化合物含量。使用ABTS法和铁还原抗氧化能力法进行体外抗氧化活性测定。
结果
墨西哥红毛丹果壳的总多酚含量为582 mg/g，通过ABTS法和铁还原抗氧化能力分析显示出明显的抗氧化活性。高效液相色谱/电喷雾电离/质谱分析鉴定出13种化合物，其中大部分属于鞣花单宁。样品中存在诃子鞣质、柯里拉京和鞣花酸；还评估了矿物质组成。
结论
在墨西哥种植的红毛丹果壳是回收具有抗氧化活性的高附加值生物活性化合物的有前景来源，这些化合物作为生物活性抗氧化剂在疾病治疗方面具有潜在应用。
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