Jantapaso Husanai, Mittraparp-Arthorn Pimonsri
Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Molecular Evolution and Computational Biology Research Unit, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Antioxidants (Basel). 2022 May 12;11(5):956. doi: 10.3390/antiox11050956.
Thailand is one of the leading exporting countries of rambutan and rambutan peels are considered as a biological waste. In this study, rambutan ( L. cv. Rong Rian) peel extracts (RPE) obtained by water extraction were analyzed for their phytochemical composition, antibacterial and antioxidant activities, and cytotoxicity. The bioactive compounds in RPE identified by GC-MS were mome inositol (35.99 mg/g), catechol (29.37 mg/g), 5-hydroxymethylfurfural (5.69 mg/g), 2-pentenal, (E)-(5.22 mg/g), acetic acid (3.69 mg/g), 1,2,3-propanetriol (3.67 mg/g), 2-furan-carboxaldehyde (2.66 mg/g), and other compounds. FT-IR analysis confirmed the presence of alcohol and phenol in the extract. Antibacterial activities of RPE against food pathogenic and spoilage bacteria showed that RPE could inhibited , . , , , , , and , with MIC values ranging between 1024 and 8192 µg/mL. The extract also showed antioxidant properties, as determined by DPPH and ABTS assays. The cytotoxicity analysis after 72 h of treatment showed the IC values at 194.97 ± 4.87, 205.92 ± 2.55, and 94.11 ± 1.33 µg/mL for L929, Vero, and MCF-7 cell lines, respectively. Therefore, this study provided a basis of knowledge of rambutan peels as an excellent source of natural bioactive compounds for various applications.
泰国是红毛丹的主要出口国之一，红毛丹果皮被视为生物废弃物。在本研究中，对通过水提取获得的红毛丹（L. cv. Rong Rian）果皮提取物（RPE）进行了植物化学成分、抗菌和抗氧化活性以及细胞毒性分析。通过气相色谱 - 质谱联用（GC - MS）鉴定出RPE中的生物活性化合物有肌醇（35.99毫克/克）、儿茶酚（29.37毫克/克）、5 - 羟甲基糠醛（5.69毫克/克）、（E） - 2 - 戊烯醛（5.22毫克/克）、乙酸（3.69毫克/克）、1,2,3 - 丙三醇（3.67毫克/克）、2 - 呋喃甲醛（2.66毫克/克）以及其他化合物。傅里叶变换红外光谱（FT - IR）分析证实提取物中存在醇类和酚类。RPE对食品致病菌和腐败菌的抗菌活性表明，RPE能够抑制 ， ， ， ， ， ，以及 ，其最低抑菌浓度（MIC）值在1024至8192微克/毫升之间。通过二苯基苦味酰基自由基（DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）测定，该提取物还表现出抗氧化特性。处理72小时后的细胞毒性分析表明，L929、Vero和MCF - 7细胞系的半数抑制浓度（IC）值分别为194.97±4.87、205.92±2.55和94.11±1.33微克/毫升。因此，本研究为红毛丹果皮作为各种应用的天然生物活性化合物的优质来源提供了知识基础。
