Department of General Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan, 450000, China.
Department of Department of Reproductive Center, Henan Provincial People's Hospital, Zhengzhou, Henan, 450000, China.
Int J Biol Macromol. 2018 Mar;108:1120-1127. doi: 10.1016/j.ijbiomac.2017.12.109. Epub 2017 Dec 21.
A complicated sulfated fucoidan, SHPPB2, was purified from Sargassum henslowianum by DEAE-cellulose 52 and Sephacryl S-300 column chromatography. Via chemical and spectral method, SHPPB2 was found to contain mannose, glucuronic acid, galactose, xylose, and fucose, in a ratio of 17.4: 13.5: 10.5: 16.8: 41.8, as well as 21.4% of sulfate. The methylation analysis demonstrated terminal, 3-, 4-, 2, 3-, and 3, 4- linked fucose, 2-, 2, 3-, 2, 4-, and 2, 4, 6- linked mannose, terminal, 4-, 6-, 2, 4-, 3, 4-, and 3, 6- linked galactose, terminal and 4- linked xylose, and 4- linked glucuronic acid. In addition, the sulfate groups were substituted on the C-2, C-3 or C-4 of 3- and 4- linked fucose, on the C-4 or C-6 of 2- linked mannose, and on C-2 or C-3 of 4- and 6- linked galactose. With the treatment of SHPPB2 in the N-methyl-N'-nitro-nitrosoguanidine (MNNG) induced gastric cancer rats, it was observed with an increased body weight, and improved immune organ indices. Furthermore, SHPPB2 could significantly promote splenocyte proliferation induced by ConA or LPS in gastric cancer rats, and improve anti-inflammatory cytokines (IL-2, IL-4, and IL-10) secretion, but reduce pro-inflammatory cytokines (IL-6 and TNF-α). Taken together, it suggested that SHPPB2 could improve immune function in gastric cancer rats. Thus, it could be explored as a potential immuno-therapy for gastric cancer treatment.
一种复杂的硫酸化褐藻糖胶 SHPPB2 从马尾藻中通过 DEAE-纤维素 52 和 Sephacryl S-300 柱层析纯化而来。通过化学和光谱方法，发现 SHPPB2 含有甘露糖、葡萄糖醛酸、半乳糖、木糖和岩藻糖，摩尔比为 17.4:13.5:10.5:16.8:41.8，以及 21.4%的硫酸基。甲基化分析表明末端、3-、4-、2-、3-和 3、4-连接的岩藻糖，2-、2、3-、2、4-和 2、4、6-连接的甘露糖，末端、4-、6-、2、4-、3、4-和 3、6-连接的半乳糖，末端和 4-连接的木糖，以及 4-连接的葡萄糖醛酸。此外，硫酸基取代了 3-和 4-连接的岩藻糖的 C-2、C-3 或 C-4，2-连接的甘露糖的 C-4 或 C-6，以及 4-和 6-连接的半乳糖的 C-2 或 C-3。用 SHPPB2 处理 N-甲基-N'-亚硝基-N-亚硝基胍（MNNG）诱导的胃癌大鼠，观察到体重增加，免疫器官指数改善。此外，SHPPB2 能显著促进胃癌大鼠脾淋巴细胞增殖，ConA 或 LPS 诱导，改善抗炎细胞因子（IL-2、IL-4 和 IL-10）分泌，但减少促炎细胞因子（IL-6 和 TNF-α）。总之，SHPPB2 能改善胃癌大鼠的免疫功能。因此，它可以作为一种潜在的胃癌免疫治疗方法进行探索。
