Hertogs Karolien, Haegeman Annelies, Schaumont Dries, Gelaude Philippe, De Zutter Lieven, Dewulf Jeroen, Heyndrickx Marc, Rasschaert Geertrui
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), 9820 Merelbeke, Belgium.
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
Pathogens. 2021 Jan 13;10(1):66. doi: 10.3390/pathogens10010066.
Biosecurity seems to be the most promising tool for control on poultry farms. A longitudinal molecular epidemiological study was performed during two production cycles, in which the broilers, the poultry house, and the environment of 10 (mixed) broiler farms were monitored weekly. Cecal droppings from the second production cycle were also used for 16S metabarcoding to study the differences in the microbiota of colonized and uncolonized flocks. Results showed that 3 out of 10 farms were positive for in the first production cycle, and 4 out of 10 were positive in the second. Broilers became colonized at the earliest when they were four weeks old. The majority of the flocks (57%) became colonized after partial depopulation. Before colonization of the flocks, was rarely detected in the environment, but it was frequently isolated from cattle and swine. Although these animals appeared to be consistent carriers of , molecular typing revealed that they were not the source of flock colonization. In accordance with previous reports, this study suggests that partial depopulation appears to be an important risk factor for introduction into the broiler house. Metabarcoding indicated that two -free flocks carried high relative abundances of in their ceca, suggesting potential competition with .
生物安全似乎是家禽养殖场控制方面最具前景的手段。在两个生产周期内开展了一项纵向分子流行病学研究，每周对10个（混合）肉鸡养殖场的肉鸡、禽舍及环境进行监测。第二个生产周期的盲肠粪便样本也用于16S宏条形码分析，以研究定殖和未定殖鸡群微生物群的差异。结果显示，10个养殖场中有3个在第一个生产周期检测到阳性，10个中有4个在第二个生产周期检测到阳性。肉鸡最早在4周龄时开始定殖。大多数鸡群（57%）在部分清栏后开始定殖。在鸡群定殖前，环境中很少检测到[具体病原体名称未给出]，但它经常从牛和猪中分离出来。尽管这些动物似乎是[具体病原体名称未给出]的持续携带者，但分子分型显示它们不是鸡群定殖的源头。与之前的报告一致，本研究表明部分清栏似乎是[具体病原体名称未给出]引入肉鸡舍的一个重要风险因素。宏条形码分析表明，两个未感染[具体病原体名称未给出]的鸡群盲肠中[具体病原体名称未给出]的相对丰度较高，表明可能与[具体病原体名称未给出]存在竞争。
