Bryant William A, Stentz Régis, Le Gall Gwenaelle, Sternberg Michael J E, Carding Simon R, Wilhelm Thomas
Centre for Integrative Systems Biology and Bioinformatics, Department of Life Sciences, Imperial College London, London, United Kingdom.
Gut Health and Food Safety Programme, Quadram Institute Bioscience, Norwich, United Kingdom.
Front Microbiol. 2017 Dec 8;8:2440. doi: 10.3389/fmicb.2017.02440. eCollection 2017.
The interactions between the gut microbiota and its host are of central importance to the health of the host. Outer membrane vesicles (OMVs) are produced ubiquitously by Gram-negative bacteria including the gut commensal . These vesicles can interact with the host in various ways but until now their complement of small molecules has not been investigated in this context. Using an untargeted high-coverage metabolomic approach we have measured the small molecule content of these vesicles in contrasting conditions to establish what role these metabolites could perform when packed into these vesicles. packs OMVs with a highly conserved core set of small molecules which are strikingly enriched with mouse-digestible metabolites and with metabolites previously shown to be associated with colonization of the murine GIT. By use of an expanded genome-scale metabolic model of and a potential host (the mouse) we have established many possible metabolic pathways between the two organisms that were previously unknown, and have found several putative novel metabolic functions for mouse that are supported by gene annotations, but that do not currently appear in existing mouse metabolic networks. The lipidome of these OMVs bears no relation to the mouse lipidome, so the purpose of this particular composition of lipids remains unclear. We conclude from this analysis that through intimate symbiotic evolution OMVs produced by are likely to have been adopted as a conduit for small molecules bound for the mammalian host .
肠道微生物群与其宿主之间的相互作用对宿主健康至关重要。外膜囊泡（OMV）由包括肠道共生菌在内的革兰氏阴性菌普遍产生。这些囊泡可以通过多种方式与宿主相互作用，但到目前为止，它们所含的小分子在这种情况下尚未得到研究。我们使用一种非靶向的高覆盖代谢组学方法，在对比条件下测量了这些囊泡的小分子含量，以确定这些代谢物包装到这些囊泡中时可能发挥的作用。[具体细菌名称]用一组高度保守的小分子包装OMV，这些小分子显著富含可被小鼠消化的代谢物以及先前显示与小鼠胃肠道定殖相关的代谢物。通过使用[具体细菌名称]的扩展基因组规模代谢模型和潜在宿主（小鼠），我们建立了两种生物体之间许多以前未知的可能代谢途径，并发现了小鼠的几种推测的新代谢功能，这些功能得到了基因注释的支持，但目前在现有的小鼠代谢网络中尚未出现。这些OMV的脂质组与小鼠脂质组无关，因此这种特定脂质组成的目的仍不清楚。我们从这项分析中得出结论，通过密切的共生进化，[具体细菌名称]产生的OMV可能已被用作运往哺乳动物宿主的小分子的管道。
