饱和脂肪酸和不饱和脂肪酸对[具体来源]外膜囊泡产生的影响。 （注：原文中“from and ”表述不完整，可能影响准确理解）
The effect of saturated and unsaturated fatty acids on the production of outer membrane vesicles from and .
作者信息
Mirjafari Tafti Zahra Sadat, Moshiri Arfa, Ettehad Marvasti Fateme, Tarashi Samira, Sadati Khalili Seyedeh Fatemeh, Motahhary Atiyyeh, Fateh Abolfazl, Vaziri Farzam, Ahmadi Badi Sara, Siadat Seyed Davar
机构信息
Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Gastrointestinal Cancer Department, Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Gastroenterol Hepatol Bed Bench. 2019 Spring;12(2):155-162.
AIM
The aim of present study is to investigate the effect of fatty acids on the outer membrane vesicles (OMVs) produced by spp.
BACKGROUND
spp. is the important member of Gut microbiota that employ OMVs production for interact with host. Besides, dietary fatty acids could influence on determination of gut microbiota composition and immune response. In this regard, we evaluated the effect of fatty acids on the growth and OMVs production of and .
METHODS
and were grown on BHI broth with and without palmitic and palmitoleic acids as saturated and unsaturated fatty acids, respectively. OMVs were extracted using multiple centrifugation and tris-ethylene diamine tetra acetic acid (EDTA)-Sodium deoxy cholate buffers. Physicochemical properties of OMVs were detected by electron microscopy (SEM), Bradford Coomassie brilliant blue assay and SDS-PAGE. Data were analyzed with One-way ANOVA using SPSS.
RESULTS
The growths of both were significantly increased by palmitic acid. Nevertheless, palmitoleic acid had no significant effect on them. Palmitic acid significantly decreased and increased the production of OMVs at low and high concentration, respectively. However, the production of OMVs was not significantly affected by palmitic acid. Although palmitoleic acid had a significant decreasing effect on the production of OMVs, it significantly increased the production of OMVs at low concentration.
CONCLUSION
In conclusion we reported that palmitic acid had a stimulatory effect on the growth of and and had a dose dependent effect on the production of OMVs. Also producing of OMVs was affected by palmitoleic acid in a dose dependent manner.
目的
本研究旨在探讨脂肪酸对某菌产生的外膜囊泡（OMVs）的影响。
背景
某菌是肠道微生物群的重要成员，其通过产生OMVs与宿主相互作用。此外，膳食脂肪酸可影响肠道微生物群组成的确定和免疫反应。在这方面，我们评估了脂肪酸对某菌和另一菌生长及OMVs产生的影响。
方法
某菌和另一菌分别在含有和不含棕榈酸和棕榈油酸（分别作为饱和脂肪酸和不饱和脂肪酸）的脑心浸液肉汤（BHI）中培养。使用多次离心和三乙烯二胺四乙酸（EDTA）-脱氧胆酸钠缓冲液提取OMVs。通过电子显微镜（SEM）、考马斯亮蓝布拉德福德法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）检测OMVs的理化性质。数据用SPSS进行单因素方差分析。
结果
棕榈酸显著增加了两菌的生长。然而，棕榈油酸对它们没有显著影响。棕榈酸在低浓度时显著降低、在高浓度时显著增加某菌OMVs的产生。然而，棕榈酸对另一菌OMVs的产生没有显著影响。尽管棕榈油酸对某菌OMVs的产生有显著的降低作用，但在低浓度时它显著增加了另一菌OMVs的产生。
结论
总之，我们报道棕榈酸对某菌和另一菌的生长有刺激作用，并且对某菌OMVs的产生有剂量依赖性影响。此外，另一菌OMVs的产生也受到棕榈油酸的剂量依赖性影响。
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