Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
Department of Microbiology, Faculty of Medicine, Kuwait University, Safat, Kuwait.
Mycoses. 2018 May;61(5):305-313. doi: 10.1111/myc.12741. Epub 2018 Jan 18.
A study of environmental distribution revealed the occurrence of Cryptococcus neoformans and C. gattii in 9% and 3%, respectively, of 611 samples investigated. C. neoformans showed the highest isolation frequency from tree trunk hollows in Delhi (31%), whereas C. gattii occurred in 12% of the samples in Delhi and 5% in Rajasthan. In addition, Cryptococcus laurentii (=Papiliotrema laurentii), C. rajasthanensis (=Papiliotrema rajasthanensis), C. podzolicus (=Saitozyma podzolica) and C. flavescens (=Papiliotrema flavescens) occurred in 0.5% each. The recovery of C. flavescens and C. podzolicus was new findings for India. One more noteworthy finding was isolation of a new yeast, recently classified as Saitozyma cassiae sp. Novo. The previous strain of this yeast came from tree bark debris in South India. Our isolates came from decayed wood inside a trunk hollow of an Acacia tree in, Bharatpur Bird Sanctuary, Rajasthan. The isolations of novel strains of Cutaneotrichosporon moniliiforme from decayed wood of a Pinus tree was another significant finding. Phenotypically, they differed from T. moniliforme by being encapsulated cells, had melanin-like pigment production and were unable to assimilate d-manitol and d-melezitose. AFLP analysis showed a distinctive banding profile vis-a-vis the reference strains of T. moniliiforme and Cryptotrichosporon anacardii.
一项环境分布研究显示，在调查的 611 个样本中，分别有 9%和 3%的样本中发现了新型隐球菌和荚膜组织胞浆菌。新型隐球菌在德里树干空洞中的分离频率最高（31%），而荚膜组织胞浆菌则分别在德里和拉贾斯坦邦的 12%和 5%的样本中出现。此外，还分别在 0.5%的样本中发现了罗伦隐球菌（=Papiliotrema laurentii）、拉贾斯坦隐球菌（=Papiliotrema rajasthanensis）、聚多曲霉（=Saitozyma podzolica）和黄曲霉（=Papiliotrema flavescens）。黄曲霉和聚多曲霉的分离是印度的新发现。另一个值得注意的发现是分离到一种新的酵母，最近被归类为 Saitozyma cassiae sp. Novo。该酵母的前一个菌株来自印度南部的树皮碎屑。我们的分离株来自拉贾斯坦邦 Bharatpur 鸟类保护区一棵金合欢树树干空洞内的腐朽木材。从一棵松树腐朽木材中分离出新型的毛孢子菌也是一个重要发现。表型上，它们与 T. moniliforme 的区别在于它们是被囊细胞，具有类似黑色素的色素产生，并且不能同化 d-甘露醇和 d-梅乐蔗糖。AFLP 分析显示，与 T. moniliforme 和 Cryptotrichosporon anacardii 的参考菌株相比，它们具有独特的带型谱。
