College of Forestry and Landscape Architecture, Guangdong Province Research Center of Woody Forage Engineering Technology, Guangdong Research and Development Centre of Modern Agriculture (Woody Forage) Industrial Technology, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China.
College of Animal Science and Technology, China Agricultural University, Beijing, China.
Microb Biotechnol. 2020 Sep;13(5):1502-1514. doi: 10.1111/1751-7915.13588. Epub 2020 May 25.
To comprehensively evaluate the fermentation performance and microbial community of corn stalks (CS) silage mixed with Neolamarckia cadamba leaves (NCL), CS were ensiled with four levels (0%, 10%, 30% and 50% of fresh weight) of NCL for 1, 7, 14, 30, 60 days in two trials. The results showed that all silages were well preserved with low pH (3.60-3.88) and ammonia nitrogen content (0.08-0.19% DM). The silage samples with NCL displayed lower (P < 0.05) acetic acid, propionic acid and ammonia nitrogen contents and lactic acid bacteria population during ensiling than control silages (100% CS). The addition of NCL also influenced the distribution of bacterial and fungal communities. Fungal diversity (Shannon's indices were 5.15-5.48 and 2.85-4.27 in trial 1 and trial 2 respectively) increased while the relative abundances of Lactobacillus, Leuconostocs, Acetobacter and two moulds (Aspergillus and Fusarium) decreased after added NCL. In summary, mixing NCL is a promising effective approach to preserve protein of CS silage and inhibit the growth of undesirable bacteria and mould, thus to improve the forage quality to some extent.
为全面评价玉米秸秆（CS）与南牡荆叶（NCL）混合青贮的发酵特性和微生物区系，本试验在 2 个批次中，采用 CS 分别与 0%、10%、30%和 50%（鲜重）NCL 混合青贮，青贮 1、7、14、30 和 60 d。结果表明，所有青贮均保持良好，pH（3.60-3.88）和氨态氮含量（0.08-0.19%DM）较低。与对照青贮（100%CS）相比，添加 NCL 的青贮在青贮过程中乙酸、丙酸和氨态氮含量以及乳酸菌数量较低（P<0.05）。NCL 的添加还影响了细菌和真菌群落的分布。真菌多样性（试验 1 和试验 2 的 Shannon 指数分别为 5.15-5.48 和 2.85-4.27）增加，而添加 NCL 后，乳杆菌属、明串珠菌属、醋杆菌属和两种霉菌（曲霉属和镰刀菌属）的相对丰度降低。综上所述，添加 NCL 是一种有前途的有效方法，可以保持 CS 青贮的蛋白质含量，抑制不良细菌和霉菌的生长，从而在一定程度上提高饲料质量。
