Patel Shweta, Jana Sarmita, Chetty Rajlakshmi, Thakore Sonal, Singh Man, Devkar Ranjitsinh
a Department of Zoology , The M.S. University of Baroda , Vadodara , India.
b School of Chemical sciences , Central University of Gujarat , Gandhinagar , India.
Drug Chem Toxicol. 2019 Jan;42(1):1-8. doi: 10.1080/01480545.2017.1413110. Epub 2017 Dec 27.
Magnetic iron oxide nanoparticles (IONs) display the ability to cross blood - brain barrier and are envisioned as diagnostic and therapeutic applications, but there are few studies on their potential embryonic toxicity in higher vertebrates. This study investigates interaction of IONs with egg albumen and its subsequent toxicity on chicken embryo. Physicochemical interactions of IONs with egg albumen revealed alterations in friccohesity and secondary structural changes due to weak Vander Waals forces. Toxicity assessment of IONs (10, 25, 50, 100, and 200 µg/ml doses) on chicken embryo accounted for 100% mortality at 200 µg/ml dose due to Fe ions overload. However, lower doses (50 and 100 µg/ml) recorded decrement in whole weights and crown-rump lengths of chicken embryo possibly due to ION-albumen interactions. Histology of brain tissue revealed degeneration of neurons (50-60%) at 10-100 µg/ml dose range of IONs. Toxicity studies of IONs with diverse animal models are needed to set a toxicity benchmark for preventing embryonic toxicity prior to its use in biomedical applications. This is the first study on toxicity assessment of IONs in chicken embryo.
磁性氧化铁纳米颗粒（IONs）具有穿越血脑屏障的能力，并被设想用于诊断和治疗，但关于其在高等脊椎动物中的潜在胚胎毒性的研究很少。本研究调查了IONs与蛋清的相互作用及其对鸡胚的后续毒性。IONs与蛋清的物理化学相互作用显示，由于弱范德华力，摩擦粘性发生改变，二级结构也发生变化。对鸡胚的IONs毒性评估（剂量为10、25、50、100和200μg/ml）表明，由于铁离子过载，200μg/ml剂量时死亡率为100%。然而，较低剂量（50和100μg/ml）的鸡胚全重和冠臀长度有所下降，这可能是由于ION-蛋清相互作用所致。脑组织组织学显示，在IONs剂量为10-100μg/ml范围内，神经元退化（50-60%）。需要对IONs进行多种动物模型的毒性研究，以便在其用于生物医学应用之前设定毒性基准，以预防胚胎毒性。这是第一项关于IONs对鸡胚毒性评估的研究。
