University of Haute-Alsace, University of Strasbourg, CNRS UMR7361, IS2M, 68057, Mulhouse, France.
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil.
Adv Healthc Mater. 2018 Apr;7(8):e1701154. doi: 10.1002/adhm.201701154. Epub 2017 Dec 28.
The proper integration of biophysical cues from the cell vicinity is crucial for cells to maintain homeostasis, cooperate with other cells within the tissues, and properly fulfill their biological function. It is therefore crucial to fully understand how cells integrate these extracellular signals for tissue engineering and regenerative medicine. Topography has emerged as a prominent component of the cellular microenvironment that has pleiotropic effects on cell behavior. This progress report focuses on the recent advances in the understanding of the topography sensing mechanism with a special emphasis on the role of the nucleus. Here, recent techniques developed for monitoring the nuclear mechanics are reviewed and the impact of various topographies and their consequences on nuclear organization, gene regulation, and stem cell fate is summarized. The role of the cell nucleus as a sensor of cell-scale topography is further discussed.
细胞周围的生物物理线索的适当整合对于细胞维持内稳态、与组织内的其他细胞合作以及正确履行其生物学功能至关重要。因此，充分了解细胞如何整合这些细胞外信号对于组织工程和再生医学至关重要。形貌已成为细胞微环境的一个突出组成部分，对细胞行为具有多效性影响。本进展报告重点介绍了对形貌感知机制的最新理解，特别强调了细胞核的作用。本文回顾了最近开发的用于监测核力学的技术，并总结了各种形貌及其对核组织、基因调控和干细胞命运的影响。进一步讨论了细胞核作为细胞尺度形貌传感器的作用。
