Abdelmegid Shaimaa, Kelton David, Caswell Jeff, Kirby Gordon
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
Microorganisms. 2020 Nov 28;8(12):1883. doi: 10.3390/microorganisms8121883.
Bovine mastitis remains a primary focus of dairy cattle disease research due to its considerable negative economic impact on the dairy industry. Subclinical mastitis (SCM), commonly caused by , lacks overt clinical signs and the diagnosis is based on bacteriological culture and somatic cell counts of milk, both of which have limitations. The main objective of this study was to identify, characterize and quantify the differential abundance of milk whey proteins from cows with SCM compared to whey from healthy cows. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled with liquid chromatography and tandem mass spectrometry, 28 high-abundant proteins were detected in whey from mastitic milk, 9 of which had host defense functions. These included acute phase proteins involved in innate immunity and antimicrobial functions (e.g., serotransferrin, complement C3, fibrinogen gamma-B chain and cathepsin B), and proteins associated with the immune response to pathogens (e.g., polymeric immunoglobulin receptor-like protein, MHC class I antigen and beta-2-microglobulin). These results provide a unique 2D map of the modulated milk proteome during mastitis. The broader importance is that the identified proteins, particularly those with host-defense biological functions, represent potential candidate biomarkers of subclinical mastitis in dairy cows.
由于牛乳腺炎对乳制品行业具有相当大的负面经济影响，它仍然是奶牛疾病研究的主要焦点。亚临床乳腺炎（SCM）通常由 引起，缺乏明显的临床症状，其诊断基于细菌培养和牛奶体细胞计数，而这两者都有局限性。本研究的主要目的是识别、表征和量化患SCM奶牛的乳清蛋白与健康奶牛乳清蛋白相比的差异丰度。使用二维差异凝胶电泳（2D-DIGE）结合液相色谱和串联质谱，在患乳腺炎牛奶的乳清中检测到28种高丰度蛋白，其中9种具有宿主防御功能。这些蛋白包括参与固有免疫和抗菌功能的急性期蛋白（如血清转铁蛋白、补体C3、纤维蛋白原γ-B链和组织蛋白酶B），以及与病原体免疫反应相关的蛋白（如聚合免疫球蛋白受体样蛋白、MHC I类抗原和β-2-微球蛋白）。这些结果提供了乳腺炎期间乳蛋白质组调节的独特二维图谱。更广泛的重要性在于，所鉴定的蛋白，特别是那些具有宿主防御生物学功能的蛋白，代表了奶牛亚临床乳腺炎的潜在候选生物标志物。
