Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Fish Shellfish Immunol. 2018 Mar;74:52-61. doi: 10.1016/j.fsi.2017.12.030. Epub 2017 Dec 25.
In the practical farming of Litopenaeus vannamei, the intensive culture system and environmental pollution usually results in a high concentration of ammonia, which brings large detrimental effects to shrimp, such as increasing the susceptibility to pathogens and even causing high mortality. We have revealed that the survival time under acute ammonia stress varied substantially among different families and obtained ammonia-tolerant (LV_T) and ammonia-sensitive (LV_S) families. In order to understand the molecular mechanism of defense against ammonia toxicity in shrimp, we performed iTRAQ LC-MS/MS proteomic analysis between LV_T and LV_S groups of L. vannamei under acute ammonia stress to identify the key proteins and pathways that play an effective role for against ammonia toxicity. By comparative proteome analysis, 202 significantly differentially proteins (DEPs) were identified in LV_T compared to LV_S, and most of the DEPs (60%) were up-regulated. Excepting for the proteins without function reporting, the meaningful finding is that 77.8% of the DEPs have been reported mainly involving in immune defense and stress tolerant in crustacean species, such as hemocyanin, Rab7, Rab GTPase, Rac1, alpha 2 macroglobulin, Bip, peroxiredoxin, Cu/Zn SOD, glutathione peroxidase, thioredoxin, calreticulin, and Elongation Factor 1-alpha, etc. These DEPs might potentially play important role in against ammonia toxicity, and it also reflected a relation between ammonia tolerance and pathogen resistance. In addition, a total of 10 significantly changed KEGG pathways were detected, and the network diagram of these KEGG pathways showed that more critical nodes were up-regulated, which involved in protein synthesis and transport, and against stress stimuli. This study provided important information for understanding the molecular mechanism of defense against ammonia toxicity in shrimp at whole protein level.
在凡纳滨对虾的集约化养殖中，密集的养殖系统和环境污染通常会导致氨浓度升高，这会给虾类带来很大的不利影响，例如增加对病原体的易感性，甚至导致高死亡率。我们已经发现，不同家系在急性氨胁迫下的存活时间有很大差异，并获得了耐氨（LV_T）和敏感氨（LV_S）家系。为了了解虾类抵御氨毒性的分子机制，我们对急性氨胁迫下的 LV_T 和 LV_S 凡纳滨对虾进行了 iTRAQ LC-MS/MS 蛋白质组学分析，以鉴定在抵御氨毒性方面发挥有效作用的关键蛋白和途径。通过比较蛋白质组学分析，在 LV_T 与 LV_S 之间鉴定出 202 个差异表达蛋白（DEPs），其中大多数 DEPs（60%）上调。除了没有功能报道的蛋白外，有意义的发现是，77.8%的 DEPs 主要涉及甲壳类动物的免疫防御和应激耐受，如血蓝蛋白、Rab7、Rab GTPase、Rac1、α2 巨球蛋白、Bip、过氧化物酶、Cu/Zn SOD、谷胱甘肽过氧化物酶、硫氧还蛋白、钙网蛋白和延伸因子 1-α等。这些 DEPs 可能在抵御氨毒性方面发挥重要作用，这也反映了氨耐受性与病原体抗性之间的关系。此外，还检测到 10 个显著变化的 KEGG 途径，这些 KEGG 途径的网络图显示，更多关键节点上调，涉及蛋白质的合成和运输以及对应激刺激的反应。本研究为在全蛋白水平上理解虾类抵御氨毒性的分子机制提供了重要信息。
