Chuang Hui-Min, Reifenberger Jeffrey G, Cao Han, Dorfman Kevin D
Department of Chemical Engineering and Materials Science, University of Minnesota-Twin Cities, 421 Washington Avenue SE, Minneapolis, Minnesota 55455, USA.
BioNano Genomics, 9640 Towne Centre Drive, Suite 100, San Diego, California 92121, USA.
Phys Rev Lett. 2017 Dec 1;119(22):227802. doi: 10.1103/PhysRevLett.119.227802. Epub 2017 Nov 29.
Using a high-throughput genome-mapping approach, we obtained circa 50 million measurements of the extension of internal human DNA segments in a 41 nm×41 nm nanochannel. The underlying DNA sequences, obtained by mapping to the reference human genome, are 2.5-393 kilobase pairs long and contain percent GC contents between 32.5% and 60%. Using Odijk's theory for a channel-confined wormlike chain, these data reveal that the DNA persistence length increases by almost 20% as the percent GC content increases. The increased persistence length is rationalized by a model, containing no adjustable parameters, that treats the DNA as a statistical terpolymer with a sequence-dependent intrinsic persistence length and a sequence-independent electrostatic persistence length.
通过高通量基因组图谱绘制方法，我们在一个41纳米×41纳米的纳米通道中获得了约5000万个关于人类内部DNA片段延伸的测量数据。通过与人类参考基因组进行比对得到的基础DNA序列长度为2.5至393千碱基对，GC含量百分比在32.5%至60%之间。利用奥戴克关于通道受限蠕虫状链的理论，这些数据表明，随着GC含量百分比的增加，DNA持久长度几乎增加了20%。通过一个不含可调参数的模型对增加的持久长度进行了合理化解释，该模型将DNA视为一种统计三元聚合物，具有序列依赖的固有持久长度和与序列无关的静电持久长度。
