Division of Biochemistry, Indian Agricultural Research Institute (IARI), New Delhi, India.
Department of Immunology and Genomic Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
Food Chem. 2018 Apr 15;245:246-253. doi: 10.1016/j.foodchem.2017.10.099. Epub 2017 Oct 20.
Radiation processing of soybean, varying in seed coat colour, was carried out at dose levels of 0.25, 0.5 and 1 kGy to evaluate their potential anti-proliferative and cytoprotective effects in an in vitro cell culture system. Irradiated and control black (Kalitur) and yellow (DS9712) soybean extracts were characterized in terms of total phenolics, flavonoids and anthocyanins, especially cyanidin-3-glucoside (C3G). Using an epithelial cell line, BEAS-2B the potential cytoprotective effects of soybean extracts were evaluated in terms of intracellular ROS levels and cell viability. The most relevant scavenging effect was found in Kalitur, with 78% decrease in ROS, which well correlated with a 33% increase in C3G after a 1 kGy dose. Results evidenced a correspondence between in vitro antioxidant activity and a potential health property of black soybean extracts, exemplifying the nutraceutical role of C3G. To our knowledge this study is the first report validating the cytoprotective effects of irradiated black soybean extracts.
对不同种皮颜色的大豆进行了辐照处理，剂量水平分别为 0.25、0.5 和 1 kGy，以评估它们在体外细胞培养系统中的潜在抗增殖和细胞保护作用。对辐照和对照的黑（Kalitur）和黄（DS9712）大豆提取物进行了总酚、类黄酮和花青素，特别是矢车菊素-3-葡萄糖苷（C3G）的特性分析。使用上皮细胞系 BEAS-2B，根据细胞内 ROS 水平和细胞活力评估了大豆提取物的潜在细胞保护作用。在 Kalitur 中发现了最相关的清除作用，ROS 减少了 78%，这与 1 kGy 剂量后 C3G 增加了 33%非常吻合。结果表明，体外抗氧化活性与黑大豆提取物的潜在健康特性之间存在对应关系，这例证了 C3G 的营养作用。据我们所知，这项研究首次验证了辐照黑大豆提取物的细胞保护作用。
