Suryadi Herman, Mun'im Abdul
Graduate Program of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, Kampus UI Depok, 16424, West Java, Indonesia.
Directorate of Traditional Medicines, Health Supplements and Cosmetics Registration, Indonesian Food and Drug Authority, Jakarta, 10560, DKI Jakarta, Indonesia.
Heliyon. 2021 Aug 19;7(8):e07825. doi: 10.1016/j.heliyon.2021.e07825. eCollection 2021 Aug.
(L.) Less. leaf has a long history of being used as a food and in traditional medicines. Although gamma irradiation is an effective decontamination method, it must be performed appropriately to preserve the bioactive constituents and biological activities of the plant. This study investigated the influence of gamma irradiation on the caffeoylquinic acid derivatives content, antioxidant capacity, and microbial burden of . leaf. Dried . leaf powder was exposed to gamma rays from cobalt-60 at the absorbed doses of 2.5, 5.0, 7.5, and 10 kGy. After a maceration of . leaf with 70% ethanol, the content of six caffeoylquinic acid derivatives (CQAs) in the extract was assayed using high-performance liquid chromatography. The antioxidant capacity of the ethanolic extract was also determined using the DPPH, ABTS, and ferric reducing antioxidant power (FRAP) methods. The total aerobic bacteria and total yeast and mold counts were investigated using the Petrifilm method at 0 and 3 months after irradiation. Doses of 5-10 kGy significantly increased the CQA level ( < 0.05). The antioxidant activity was enhanced significantly at 2.5-10 kGy ( < 0.05). Doses of 2.5-10 kGy also effectively reduced the microbial load ( < 0.05). Among the irradiation doses, 10 kGy showed the best results. Thus, gamma irradiation at 10 kGy is useful in increasing CQA content and antioxidant capacity as well as reducing the microbial load of . leaf.
（L.）Less.叶作为食物和传统药物使用已有很长历史。尽管伽马射线辐照是一种有效的去污方法，但必须适当进行以保留植物的生物活性成分和生物活性。本研究调查了伽马射线辐照对（L.）Less.叶中咖啡酰奎宁酸衍生物含量、抗氧化能力和微生物负荷的影响。将干燥的（L.）Less.叶粉末暴露于来自钴 - 60的伽马射线，吸收剂量分别为2.5、5.0、7.5和10千戈瑞。用70%乙醇浸渍（L.）Less.叶后，使用高效液相色谱法测定提取物中六种咖啡酰奎宁酸衍生物（CQAs）的含量。还使用DPPH、ABTS和铁还原抗氧化能力（FRAP）方法测定乙醇提取物的抗氧化能力。在辐照后0个月和3个月，使用Petrifilm方法调查总需氧菌以及总酵母菌和霉菌数量。5 - 10千戈瑞的剂量显著提高了CQAs水平（P < 0.05）。在2.5 - 10千戈瑞时抗氧化活性显著增强（P < 0.05）。2.5 - 10千戈瑞的剂量也有效降低了微生物负荷（P < 0.05）。在所有辐照剂量中，10千戈瑞显示出最佳结果。因此，10千戈瑞的伽马射线辐照有助于提高（L.）Less.叶的CQAs含量和抗氧化能力以及降低微生物负荷。
