Department of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
Department for Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia.
Food Chem Toxicol. 2018 Feb;112:118-125. doi: 10.1016/j.fct.2017.12.044. Epub 2017 Dec 26.
Chemical composition and antioxidative, genotoxic and cytotoxic potential of essential oil (EO) and post-distillation waste (PDW) of Serbian Juniperus communis L. var. saxatilis Pall. was studied in human lung carcinoma (A549) and normal lung fibroblast (MRC-5) cells. GC-MS analysis identified 93.95% of total EO content and determined α-pinen as a dominant component (23.61%). LC-MS/MS analysis of PDW pointed at rutin (12.2 mg g) and quinic acid (11.1 mg g) as the most abundant. Antioxidativity of PDW was strong in DPPH (IC was 5.27 μg mL), and moderate in TBA and FRAP assays. Both substances were more cytotoxic to A549 than to MRC-5 cells. Obtained IC values were 69.4 μg mL and 120 μg mL for EO, and 1.27 mg mL and 2.86 mg mL for PDW, respectively. PDW was genotoxic (0.3 mg mL and 1 mg mL in A549 and MRC-5 cells, respectively) and induced apoptosis and arrested cell cycle in G2/M phase in A549 cells (0.3 mg mL). In mixtures with doxorubicin cytotoxicity of EO and PDW increased, and combination index values (0.12-0.18) revealed clear synergistic effect, stronger in cancer cells. This indicates that J. communis var. saxatilis could decrease the chemotherapeutic doses of doxorubicin, potentially reducing its side effects.
研究了塞尔维亚桧柏（Juniperus communis L. var. saxatilis Pall.）精油（EO）和蒸馏后废物（PDW）的化学成分以及抗氧化、遗传毒性和细胞毒性潜力，在人肺癌（A549）和正常肺成纤维细胞（MRC-5）中进行。GC-MS 分析确定了 EO 总含量的 93.95%，并确定 α-蒎烯为主要成分（23.61%）。PDW 的 LC-MS/MS 分析表明芦丁（12.2 mg g）和奎宁酸（11.1 mg g）的含量最丰富。PDW 在 DPPH 中具有很强的抗氧化性（IC 为 5.27μg mL），在 TBA 和 FRAP 测定中具有适度的抗氧化性。两种物质对 A549 的细胞毒性均强于 MRC-5 细胞。EO 的 IC 值分别为 69.4μg mL 和 120μg mL，PDW 的 IC 值分别为 1.27mg mL 和 2.86mg mL。PDW 具有遗传毒性（在 A549 和 MRC-5 细胞中分别为 0.3 mg mL 和 1 mg mL），并在 A549 细胞中诱导细胞凋亡和将细胞周期阻滞在 G2/M 期（0.3 mg mL）。在与阿霉素的混合物中，EO 和 PDW 的细胞毒性增加，且联合指数值（0.12-0.18）表明存在明显的协同作用，在癌细胞中更强。这表明 J. communis var. saxatilis 可降低阿霉素的化疗剂量，可能降低其副作用。
