Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
Department of Food Science, International University of Korea, Jinju, Republic of Korea.
Food Chem Toxicol. 2018 Feb;112:150-156. doi: 10.1016/j.fct.2017.12.045. Epub 2017 Dec 26.
Platycodon grandiflorum contains triterpenoid saponins, such as platycodin D and platyconic acid A, and acts as a multifold nutritious compound. Our previous research demonstrated that Platycodon grandiflorum-derived saponin (PS) improves high-fat diet-induced non-alcoholic steatohepatitis and inhibits osteoclast differentiation. The pivotal effects of PS on inflammatory mechanism were suppressed NF-κB and matrix metalloproteinase (MMPs). However, the effects of PS on skeletal muscle damage remain unknown. Therefore, we investigated whether PS protects against eccentric exercise-induced muscle damage. A significant reduction in eccentric exercise-induced muscle damage area and muscle damage related to the level of NF-κB p65 by PS was associated with the downregulation of ERK/p38/SMAD signaling. Eccentric exercise caused muscle damage by increasing the serum lactate dehydrogenase (LDH), creatinine kinase (CK) and C-related protein level. The serum LDH, CK and C-related protein level was significantly lower in the PS supplementation group compared with the control group. Moreover, PS was inhibited MMP-1, MMP-2 and MMP-9. PS protects against eccentric exercise-induced muscle damage. Together, these results provide a novel perspective on the biological function of PS against muscle damage.
桔梗含有三萜皂苷，如远志酸 D 和远志酸 A，是一种具有多种营养成分的化合物。我们之前的研究表明，桔梗皂苷（PS）可改善高脂肪饮食诱导的非酒精性脂肪性肝炎，并抑制破骨细胞分化。PS 对炎症机制的关键作用是抑制 NF-κB 和基质金属蛋白酶（MMPs）。然而，PS 对骨骼肌损伤的影响尚不清楚。因此，我们研究了 PS 是否可以预防离心运动引起的肌肉损伤。PS 通过下调 ERK/p38/SMAD 信号通路，显著减少离心运动引起的肌肉损伤面积和与 NF-κB p65 水平相关的肌肉损伤。离心运动通过增加血清乳酸脱氢酶（LDH）、肌酸激酶（CK）和 C 反应蛋白水平引起肌肉损伤。PS 补充组的血清 LDH、CK 和 C 反应蛋白水平明显低于对照组。此外，PS 抑制 MMP-1、MMP-2 和 MMP-9。PS 可预防离心运动引起的肌肉损伤。综上所述，这些结果为 PS 对抗肌肉损伤的生物学功能提供了新的视角。
