Institute of Pathology of the Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 33, 04103, Leipzig, Germany.
Clinic for Ruminants and Swine of the Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 11, 04103, Leipzig, Germany.
Mycotoxin Res. 2018 May;34(2):99-106. doi: 10.1007/s12550-017-0304-z. Epub 2017 Dec 29.
In order to evaluate the influence of deoxynivalenol (DON) on histomorphological and immunohistochemical parameters in the development of porcine fetuses, five pregnant sows were fed a control diet (0.15 mg DON/kg diet) and seven sows a contaminated diet (4.42 mg DON/kg diet) between days 35 and 70 of gestation. On day 70, fetuses were delivered by caesarean section and sows and fetuses were euthanized. Tissue samples of three fetuses from each sow were collected, fixed in formalin, and processed routinely for light microscopy and immunohistochemistry. At necropsy, no macroscopic lesions were observed in any organ of the fetuses. Histomorphological, immunohistochemical, and morphometrical parameters of the immune system, liver, and intestinal tract were examined. The following antibodies were used in the liver, spleen, lymph nodes, thymus, gut, and bone marrow to compare control- and DON-treated animals: (I) CD3 and CD79a (T and B lymphocytes differentiation); (II) myeloid/histiocyte antigen 387 (MAC) (identification of macrophages); (III) Ki-67 Antigen (Ki-67) (proliferation marker); (IV) p-p-38 mitogen-activated protein kinases (p-p38 MAPK) as well as caspase-3 (cas3) and caspase-9 (cas9) (enzymes of apoptosis cascade); (V) tumor necrosis factor-alpha (TNFα) (immune-related protein). The results of the study show that exposure of pregnant sows with DON between gestation days 35 and 70 causes no pathomorphologically or immunohistochemically detectable alterations in all fetal organs examined.
为了评估脱氧雪腐镰刀菌烯醇（DON）对猪胎儿发育过程中组织形态学和免疫组织化学参数的影响，将 5 头怀孕母猪喂食对照饲料（0.15mg DON/kg 饲料），将 7 头母猪喂食污染饲料（4.42mg DON/kg 饲料），在妊娠第 35 天至第 70 天期间进行。第 70 天，通过剖腹产分娩胎儿，然后对母猪和胎儿实施安乐死。从每头母猪的 3 只胎儿中采集组织样本，用福尔马林固定，常规进行光镜和免疫组织化学检查。剖检时，未观察到任何胎儿器官的宏观病变。检查了免疫系统、肝脏和肠道的组织形态学、免疫组织化学和形态计量学参数。在肝脏、脾脏、淋巴结、胸腺、肠道和骨髓中使用了以下抗体来比较对照和 DON 处理的动物：（I）CD3 和 CD79a（T 和 B 淋巴细胞分化）；（II）髓细胞/组织细胞抗原 387（MAC）（巨噬细胞鉴定）；（III）Ki-67 抗原（Ki-67）（增殖标志物）；（IV）p-p-38 丝裂原活化蛋白激酶（p-p38 MAPK）以及 caspase-3（cas3）和 caspase-9（cas9）（细胞凋亡级联中的酶）；（V）肿瘤坏死因子-α（TNFα）（免疫相关蛋白）。研究结果表明，在妊娠第 35 天至第 70 天期间，用 DON 暴露怀孕母猪不会导致所有检查的胎儿器官发生形态学或免疫组织化学上可检测到的改变。
