College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, Hunan 410082, China.
College of Environmental Science and Engineering, Hunan University and Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, Hunan 410082, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang 310018, China.
Bioresour Technol. 2018 Mar;251:274-279. doi: 10.1016/j.biortech.2017.12.058. Epub 2017 Dec 20.
Microalgae Coelastrella sp. could remove nutrients from anaerobically digested swine wastewater (ADSW) effectively, while its responses to the stress of Cu(II) were not well understood. In this paper, nutrients removal and growth of Coelastrella sp. were investigated at the presence of Cu(II) in ADSW. Results showed ammonium nitrogen concentration in ADSW decreased with culturing duration, while increased with an increased Cu(II) concentration. Total phosphorous concentration decreased with time, while did not drop in 4 days at Cu(II) concentration ≥1.0 mg/L. Microalgal growth was inhibited at all the Cu(II) concentrations, and ceased in about 6-8 days at Cu(II) concentration ≥1.0 mg/L. With an increased Cu(II) concentration, the contents of chlorophyll a and proteins decreased, those of malondialdehyde and superoxide dismutase, and ratios of octadecanoic acid (C18:0), hexadecanoic acid (C16:0) and octadecenoic acid (C18:1) to fatty acids in Coelastrella sp. increased, while octadecatrienoic acid (C18:3) gradually disappeared.
微藻小球藻可以有效地从厌氧消化猪废水中（ADSW）去除营养物质，但其对 Cu(II) 胁迫的反应还不太清楚。在本文中，研究了 Cu(II) 在 ADSW 中存在时小球藻的营养物质去除和生长情况。结果表明，ADSW 中的氨氮浓度随培养时间的延长而降低，而随 Cu(II)浓度的增加而增加。总磷浓度随时间降低，但在 Cu(II)浓度≥1.0mg/L 时 4 天内未下降。小球藻的生长在所有 Cu(II)浓度下均受到抑制，在 Cu(II)浓度≥1.0mg/L 时约 6-8 天停止。随着 Cu(II)浓度的增加，小球藻中的叶绿素 a 和蛋白质含量减少，丙二醛和超氧化物歧化酶的含量增加，以及脂肪酸中十八烷酸（C18:0）、十六烷酸（C16:0）和十八烯酸（C18:1）与脂肪酸的比例增加，而十八碳三烯酸（C18:3）逐渐消失。
