Department of Agronomy, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
Department of Agronomy, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China.
Plant Physiol Biochem. 2018 Feb;123:319-330. doi: 10.1016/j.plaphy.2017.12.032. Epub 2017 Dec 24.
In our previous study, Tibetan wild barley (Hordeum spontaneum L.) has been found to be rich in the elite accessions with strong abiotic stress tolerance, including salt stress tolerance. However, the molecular mechanism of salt tolerance underlying the wild barley remains to be elucidated. In this study, two Tibetan wild barley accessions, XZ26 (salt-tolerant) and XZ169 (salt-sensitive), were used to investigate ionomic, metabolomic and proteomic responses in roots when exposed to 0, 200 (moderate) and 400 mM (high) salinity. XZ26 showed stronger root growth and maintained higher K concentrations when compared with XZ169 under moderate salinity, while no significant difference was found between the two accessions under high salinity. A total of 574 salt-regulated proteins and 153 salt-regulated metabolites were identified in the roots of both accessions based on quantitative proteomic (iTRAQ methods) and metabolomic (GC-TOF/MS) analysis. XZ26 developed its root adaptive strategies mainly by accumulating more compatible solutes such as proline and inositol, acquiring greater antioxidant ability to cope with ROS, and consuming less energy under salt stress for producing biomass. These findings provide a better understanding of molecular responses of root adaptive strategies to salt stress in the wild barley.
在我们之前的研究中，发现西藏野生大麦（Hordeum spontaneum L.）富含具有强抗逆性的优良品种，包括耐盐性。然而，野生大麦耐盐性的分子机制仍有待阐明。在这项研究中，我们使用了两个西藏野生大麦品种 XZ26（耐盐）和 XZ169（盐敏感），来研究在 0、200（中度）和 400mM（高）盐胁迫下根系的离子组、代谢组和蛋白质组的响应。与 XZ169 相比，XZ26 在中度盐胁迫下表现出更强的根生长和更高的 K 浓度，而在高盐胁迫下，两个品种之间没有发现显著差异。基于定量蛋白质组学（iTRAQ 方法）和代谢组学（GC-TOF/MS）分析，在两个品种的根系中共鉴定出 574 个盐调节蛋白和 153 个盐调节代谢物。XZ26 主要通过积累更多的相容性溶质（如脯氨酸和肌醇）、获得更大的抗氧化能力来应对 ROS 以及在产生生物量时消耗更少的能量来发展其根适应策略。这些发现为深入了解野生大麦根系适应盐胁迫的分子响应提供了更好的认识。
