Patini Romeo, Gallenzi Patrizia, Spagnuolo Gianrico, Cordaro Massimo, Cantiani Monica, Amalfitano Adriana, Arcovito Alessandro, Callà Cinzia, Mingrone Gertrude, Nocca Giuseppina
Institute of Dentistry, Università Cattolica del Sacro Cuore, Largo Francesco Vito - 00168 Rome , Italy.
Department of Neurosciences, Reproductive and Odontostomatological Sciences, Università di Napoli Federico II, via S. Pansini, 5-80131 Naples, Italy.
Open Dent J. 2017 Dec 12;11:621-627. doi: 10.2174/1874210601711010621. eCollection 2017.
Metabolic syndrome (MetS) is associated with an increased risk of periodontitis even if the mechanism is unknown. Since both MetS and periodontitis are characterized by an alteration of inflammation status, the aim of this pilot study was to determine if differences in ROS metabolism of phagocytes isolated from (A) patients with MetS, (B) patients with both MetS and mild periodontitis, (C) healthy subjects and (D) normal weight subjects with mild periodontitis, were present.
ROS metabolism was studied by a Chemiluminescence (CL) technique: the system was made up of luminol (100 nmol/L) and cells (1 × 10) in the presence or absence of stimulus constituted by opsonized zymosan (0.5 mg). The final volume (1.0 mL) was obtained using modified KRP buffer. ROS production was measured at 25°C for 2 h, using an LB 953 luminometer (Berthold, EG & G Co, Germany). All the experiments were performed in triplicate.
All results are mean ± standard deviation (SD). The group of means was compared by the analysis of variance "(ANOVA)". A value of < 0.05 was considered significant.
Results showed that basal ROS production (both from PMNs and from PBMs) of groups A, B and D was increased with respect to that obtained from group C ( <0.05).
These results are congruent with literature data, although the actual clinical relevance of the phenomenon remains to be evaluated.
代谢综合征（MetS）与牙周炎风险增加相关，但其机制尚不清楚。由于MetS和牙周炎均以炎症状态改变为特征，本初步研究的目的是确定从以下人群分离的吞噬细胞的活性氧（ROS）代谢是否存在差异：（A）MetS患者；（B）患有MetS和轻度牙周炎的患者；（C）健康受试者；（D）体重正常且患有轻度牙周炎的受试者。
采用化学发光（CL）技术研究ROS代谢：该系统由鲁米诺（100 nmol/L）和细胞（1×10）组成，在存在或不存在由调理酵母聚糖（0.5 mg）构成的刺激的情况下进行。使用改良的KRP缓冲液获得最终体积（1.0 mL）。使用LB 953发光计（德国贝托尔德公司，EG&G）在25℃下测量ROS产生2小时。所有实验均重复进行三次。
所有结果均以平均值±标准差（SD）表示。通过方差分析（ANOVA）比较均值组。P<0.05被认为具有统计学意义。
结果显示，与C组相比，A、B和D组的基础ROS产生（来自中性粒细胞和外周血单核细胞）均增加（P<0.05）。
这些结果与文献数据一致，尽管该现象的实际临床相关性仍有待评估。
