Zhao Huan, Qiu Tian, Guo Huiqin, Ying Jianming, Li Junling, Zhang Zhihui
Department of Pathology, National Cancer Center, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Department of Oncology, National Cancer Center, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Oncotarget. 2017 Nov 20;8(63):106685-106692. doi: 10.18632/oncotarget.22530. eCollection 2017 Dec 5.
The detection of and mutations of metastatic lung adenocarcinoma using liquid-based cytology suspension routine specimens from fine-needle aspiration remains controversial.
The DNA of all specimens was extracted and real time PCR was performed successfully. The rate of and mutations was 37.7% (58/154) and 5.8% (9/154), respectively. mutation rate was significantly higher in females than that in males (47.8% vs. 29.4%, = 0.019). There were no significant differences among different age groups or different tumor sites. These results of and mutations using LBC specimens were consistant with the tissue samples. In 30 patients treated with tyrosine kinase inhibitors, complete response, partial response, stable disease and progress disease was observed in 2, 10, 13 and 5 patients, respectively.
Liquid-based cytology specimen is reliable and can be an alternative source for the detection of and mutations.
154 fine-needle aspiration cytologic samples were obtained from patients with metastatic lung adenocarcinoma. The specimens included 21 cases of mediastinal lymph node 123 cases of neck nodules and 10 cases of subcutaneous nodules. After the diagnosis and count of tumor cells performed by cytopathologists, liquid-based cytology specimens with sufficient tumor cells were used for and testing using real-time PCR.
利用细针穿刺获得的液基细胞学悬浮常规标本检测转移性肺腺癌的 和 突变仍存在争议。
成功提取了所有标本的DNA并进行了实时PCR。 和 突变率分别为37.7%（58/154）和5.8%（9/154）。女性的 突变率显著高于男性（47.8%对29.4%， = 0.019）。不同年龄组或不同肿瘤部位之间无显著差异。使用液基细胞学标本检测 和 突变的这些结果与组织样本一致。在30例接受酪氨酸激酶抑制剂治疗的患者中，分别有2例、10例、13例和5例观察到完全缓解、部分缓解、病情稳定和病情进展。
液基细胞学标本可靠，可作为检测 和 突变的替代来源。
从转移性肺腺癌患者中获取154份细针穿刺细胞学样本。标本包括21例纵隔淋巴结、123例颈部结节和10例皮下结节。在细胞病理学家对肿瘤细胞进行诊断和计数后，将含有足够肿瘤细胞的液基细胞学标本用于通过实时PCR检测 和 。
