Fischer W, Heller T, Herrmann E, Schreiber D
Institut für Pathologische Anatomie, Medizinischen Akademie Erfurt, DDR.
Zentralbl Allg Pathol. 1989;135(1):33-41.
A simple method is described in this paper for the production of a polyclonal antiserum against GFAP. The antiserum was tested on 212 primary brain tumours which had been selected from biopsy and autopsy material of the Institute of Pathological Anatomy at the Medical Academy of Erfurt, GDR. 52 of 81 astrocytomas (64%) and 26 of 47 glioblastomas (55%) gave GFAP-positive results. GFAP-negative responses were primarily recorded from tumours with severe anaplasia. GFAP was found in all 22 ependymomas tested. Epithelioid ependymomas, however, exhibited lower immunological reactions than tanycytic variants. Isomorphic oligodendrogliomas, meningiomas, medulloblastomas, and brain metastases of carcinomas were GFAP-negative. The possibility is discussed in some detail of falsely negative results on account of too little biopsy material or insufficient fixation of tumour tissue.
本文描述了一种制备抗胶质纤维酸性蛋白（GFAP）多克隆抗血清的简单方法。该抗血清在212例原发性脑肿瘤上进行了检测，这些肿瘤选自德意志民主共和国爱尔福特医学院病理解剖研究所的活检和尸检材料。81例星形细胞瘤中有52例（64%）、47例胶质母细胞瘤中有26例（55%）呈现GFAP阳性结果。GFAP阴性反应主要见于间变严重的肿瘤。在所有检测的22例室管膜瘤中均发现了GFAP。然而，上皮样室管膜瘤的免疫反应低于室管膜细胞型变体。同形性少突胶质细胞瘤、脑膜瘤、髓母细胞瘤和癌的脑转移瘤均为GFAP阴性。文中详细讨论了因活检材料过少或肿瘤组织固定不足而导致假阴性结果的可能性。
