van der Meulen J D, Houthoff H J, Ebels E J
Neuropathol Appl Neurobiol. 1978 May-Jun;4(3):177-90. doi: 10.1111/j.1365-2990.1978.tb00534.x.
The presence of GFA protein, an astrocyte specific antigen, was investigated in surgical biopsies of ninety-five gliomas using an immunoperoxidase detection method. In some cases electron microscopy was added and also in one tumour immunoelectron microscopy. The results were correlated with the histological classification and grading of the tumours. In astrocytomas, the number of GFA positive cells decreased with increasing malignancy. Some malignant oligodendrogliomas surprisingly showed GFA in oligodendroglial and gemistocytic tumour cells. Thus, transition from oligodendroglial into astrocytic tumour cells seems to occur. In the mixed astrocytoma/oligodendrogliomas, theoli godendroglioma areas were negative. Subependymomas were strongly GFA positive, whereas ependymomas and medulloblastomas were negative. The presence or absence of GFA seems to contribute a 'hard' criterion for classifying gliomas and may be helpful in grading them according to the degree of malignancy.
利用免疫过氧化物酶检测方法，对95例神经胶质瘤手术活检标本中星形胶质细胞特异性抗原——胶质纤维酸性蛋白（GFA蛋白）的存在情况进行了研究。部分病例还进行了电子显微镜检查，其中1例肿瘤进行了免疫电子显微镜检查。研究结果与肿瘤的组织学分类及分级相关。在星形细胞瘤中，GFA阳性细胞数量随恶性程度增加而减少。一些恶性少突胶质细胞瘤在少突胶质细胞和肥胖型星形细胞瘤细胞中意外地显示出GFA。因此，似乎发生了从少突胶质细胞向星形细胞瘤细胞的转变。在混合性星形细胞瘤/少突胶质细胞瘤中，少突胶质细胞瘤区域为阴性。室管膜下瘤GFA呈强阳性，而室管膜瘤和髓母细胞瘤为阴性。GFA的有无似乎为神经胶质瘤的分类提供了一个“硬性”标准，并且可能有助于根据恶性程度对其进行分级。
