Ishikawa Aoi, Ohtsuki Sae, Yamada Shigehito, Uwabe Chigako, Imai Hirohiko, Matsuda Tetsuya, Takakuwa Tetsuya
Human Health Science, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan.
Congenital Anomaly Research Center, Graduate School of Medicine, Kyoto University, Kyoto, 606-8501, Japan.
Anat Rec (Hoboken). 2018 Apr;301(4):563-570. doi: 10.1002/ar.23764. Epub 2018 Jan 18.
The inner ear is a very complicated structure, composed of a bony labyrinth (otic capsule; OC), membranous labyrinth, with a space between them, named the periotic labyrinth or periotic space. We investigated how periotic tissue fluid spaces covered the membranous labyrinth three-dimensionally, leading to formation of the periotic labyrinth encapsulated in the OC during human fetal development. Digital data sets from magnetic resonance images and phase-contrast X-ray tomography images of 24 inner ear organs from 24 human fetuses from the Kyoto Collection (fetuses in trimesters 1 and 2; crown-rump length: 14.4-197 mm) were analyzed. The membranous labyrinth was morphologically differentiated in samples at the end of the embryonic period (Carnegie stage 23), and had grown linearly to more than eight times in size during the observation period. The periotic space was first detected at the 35-mm samples, around the vestibule and basal turn of the cochlea, which elongated rapidly to the tip of the cochlea and semicircular ducts, successively, and almost covered the membranous labyrinth at the 115-mm CRL stage or later. In those samples, several ossification centers were detected around the space. This article thus demonstrated that formation of the membranous labyrinth, periotic space (labyrinth), and ossification of the OC occurs successively, according to an intricate timetable. Anat Rec, 301:563-570, 2018. © 2018 Wiley Periodicals, Inc.
内耳是一个非常复杂的结构，由骨迷路（耳囊；OC）、膜迷路组成，它们之间有一个间隙，称为耳周迷路或耳周间隙。我们研究了耳周组织液间隙如何在三维空间中覆盖膜迷路，从而在人类胎儿发育过程中导致包裹在OC中的耳周迷路形成。分析了来自京都收藏的24例人类胎儿（妊娠1期和2期胎儿；顶臀长：14.4 - 197毫米）的24个内耳器官的磁共振图像和相衬X射线断层扫描图像的数字数据集。膜迷路在胚胎期末期（卡内基阶段23）的样本中形态分化，并在观察期内线性生长至超过原来大小的八倍。耳周间隙首先在35毫米样本中被检测到，位于前庭和耳蜗基底转周围，随后迅速延伸至耳蜗尖端和半规管，并在顶臀长115毫米阶段或更晚时几乎覆盖了膜迷路。在这些样本中，在间隙周围检测到几个骨化中心。因此，本文证明了膜迷路、耳周间隙（迷路）的形成以及OC的骨化是按照一个复杂的时间表相继发生的。《解剖学记录》，301:563 - 570，2018年。© 2018威利期刊公司。
