Korea Institute of Oriental Medicine, 70 Cheomdan-Ro, Dong-Gu, Daegu 41062, Korea.
Nutrients. 2017 Dec 25;10(1):20. doi: 10.3390/nu10010020.
We investigated the effects of a ethanol extract (LJE) on nonalcoholic fatty liver disease (NAFLD). An in vitro model of hepatic steatosis was treated with 1 mM free fatty acid (FFA) in HepG2 cells. An in vivo NAFLD model was established using C57BL/6 mice fed a high-fat diet (HFD) and administered LJE (100 or 200 mg/kg) orally for 14 weeks. LJE treatment suppressed lipid accumulation and intracellular triglyceride levels significantly in a concentration-dependent manner in HepG2 cells. Moreover, LJE significantly reduced the expression of sterol regulatory element binding protein 1-c, and its downstream genes, which are associated with lipogenesis, in HepG2 cells. In HFD-fed mice, LJE treatment decreased body weight significantly and decreased serum alanine transaminase levels to normal values, concurrent with a decrease in hepatic lipid accumulation. Furthermore, LJE supplementation ameliorated insulin sensitivity by decreasing serum glucose and insulin levels. LJE improved hepatic steatosis by increasing the expression of phosphorylated AMP-activated protein kinase and peroxisome proliferator-activated receptor-α in HFD-fed mice and FFA-treated HepG2 cells. The results suggested that LJE might be a potential therapeutic agent to treat NAFLD.
我们研究了乙醇提取物（LJE）对非酒精性脂肪性肝病（NAFLD）的影响。用 1mM 游离脂肪酸（FFA）处理 HepG2 细胞，建立体外肝脂肪变性模型。用高脂饮食（HFD）喂养 C57BL/6 小鼠并口服 LJE（100 或 200mg/kg）14 周，建立体内 NAFLD 模型。LJE 处理以浓度依赖的方式显著抑制 HepG2 细胞中的脂质积累和细胞内甘油三酯水平。此外，LJE 还显著降低了与脂肪生成相关的固醇调节元件结合蛋白 1-c 及其下游基因的表达。在 HFD 喂养的小鼠中，LJE 处理显著降低了体重，并使血清丙氨酸转氨酶水平降至正常，同时肝脂质积累减少。此外，LJE 通过降低血清葡萄糖和胰岛素水平改善胰岛素敏感性。LJE 通过增加 HFD 喂养小鼠和 FFA 处理的 HepG2 细胞中磷酸化 AMP 激活蛋白激酶和过氧化物酶体增殖物激活受体-α的表达来改善肝脂肪变性。结果表明，LJE 可能是治疗 NAFLD 的潜在治疗剂。
