用于追踪单个细胞的三维侧视内镜显微镜。
Three-dimensional side-view endomicroscope for tracking individual cells .
作者信息
Duan Xiyu, Li Haijun, Wang Fa, Li Xue, Oldham Kenn R, Wang Thomas D
机构信息
Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI 48109, USA.
These authors contributed equally to this work.
出版信息
Biomed Opt Express. 2017 Nov 13;8(12):5533-5545. doi: 10.1364/BOE.8.005533. eCollection 2017 Dec 1.
Abstract
We demonstrate a side-view endomicroscope using a monolithic 3-axis scanner placed in the post-objective position that performs either tilt or piston motion to achieve either optical scan angles >10° or large vertical displacements, respectively. This configuration allows for scaling down of instrument dimensions for high maneuverability and accurate positioning in vivo. Images exceeded either 700 × 600 μm in the horizontal plane or vertical depths of 200 μm. Resolution of 1.19 and 3.46 μm was obtained in the horizontal and oblique planes, respectively. Optical sections were collected from dysplastic colonic epithelium in vivo in mice that express tdTomato at 10 Hz to visualize individual cells.
摘要
我们展示了一种侧视内窥镜，它使用置于物镜后位置的整体式三轴扫描仪，该扫描仪分别执行倾斜或活塞运动，以实现大于10°的光学扫描角度或较大的垂直位移。这种配置允许缩小仪器尺寸，以实现高机动性和体内精确定位。在水平面上图像尺寸超过700×600μm，垂直深度为200μm。在水平和倾斜平面上分别获得了1.19μm和3.46μm的分辨率。从体内表达tdTomato的小鼠发育异常结肠上皮中以10Hz的频率采集光学切片，以可视化单个细胞。
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