用于体内重复成像的微型侧视双轴共聚焦内窥镜
Miniature side-view dual axes confocal endomicroscope for repetitive in vivo imaging.
作者信息
Shirazi Ahmad, Sahraeibelverdi Tayebeh, Lee Miki, Li Haijun, Yu Joonyoung, Jaiswal Sangeeta, Oldham Kenn R, Wang Thomas D
机构信息
Division of Integrative Systems and Design, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
出版信息
Biomed Opt Express. 2023 Jul 26;14(8):4277-4295. doi: 10.1364/BOE.494210. eCollection 2023 Aug 1.
A side-view dual axes confocal endomicroscope is demonstrated that can be inserted repetitively in hollow organs of genetically engineered mice for in vivo real-time imaging in horizontal and vertical planes. Near infrared (NIR) excitation at λ = 785 nm was used. A monolithic 3-axis parametric resonance scan mirror was fabricated using micro-electro-mechanical systems (MEMS) technology to perform post-objective scanning in the distal end of a 4.19 mm diameter instrument. Torsional and serpentine springs were designed to "switch" the mode of imaging between vertical and horizontal planes by tuning the actuation frequency. This system demonstrated real-time in-vivo images in horizontal and vertical planes with 310 µm depth and 1.75 and 7.5 µm lateral and axial resolution. Individual cells and discrete mucosal structures could be identified.
展示了一种侧视双轴共聚焦内窥镜，其可重复插入基因工程小鼠的中空器官中，用于在水平和垂直平面进行体内实时成像。使用了波长λ = 785 nm的近红外（NIR）激发。采用微机电系统（MEMS）技术制造了一个单片三轴参数共振扫描镜，以在直径4.19 mm的仪器远端进行物镜后扫描。设计了扭转弹簧和蛇形弹簧，通过调整驱动频率在垂直和水平平面之间“切换”成像模式。该系统展示了水平和垂直平面的体内实时图像，深度为310 µm，横向和轴向分辨率分别为1.75 µm和7.5 µm。可以识别单个细胞和离散的黏膜结构。
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