Institute of Cytology and Genetics, SB RAS, Novosibirsk, 630090, Russia.
Novosibirsk State University, Novosibirsk, 630090, Russia.
BMC Plant Biol. 2017 Dec 28;17(Suppl 2):251. doi: 10.1186/s12870-017-1193-1.
The characterization of major resistance genes (R genes) in the potato remains an important task for molecular breeding. However, R genes are rapidly evolving and frequently occur in genomes as clusters with complex structures, and their precise mapping and identification are complicated and time consuming.
Comparative analysis of root transcriptomes of Solanum phureja genotypes with contrasting resistance to Globodera rostochiensis revealed a number of differentially expressed genes. However, compiling a list of candidate R genes for further segregation analysis was hampered by their scarce annotation. Nevertheless, combination of transcriptomic analysis with data on predicted potato NBS-LRR-encoding genes considerably improved the quality of the results and provided a reasonable number of candidate genes that provide S. phureja with strong resistance to the potato golden cyst nematode.
Combination of comparative analyses of tissue-specific transcriptomes in resistant and susceptible genotypes may be used as an approach for the rapid identification of candidate potato R genes for co-segregation analysis and may be used in parallel with more sophisticated studies based on genome resequencing.
马铃薯主要抗性基因（R 基因）的特征仍然是分子育种的重要任务。然而，R 基因进化迅速，经常以复杂结构的簇形式存在于基因组中，其精确的定位和鉴定既复杂又耗时。
对具有不同抗根结线虫Globodera rostochiensis 能力的Solanum phureja 基因型的根转录组进行比较分析，揭示了许多差异表达的基因。然而，由于候选 R 基因注释稀少，难以将其列成候选基因表，用于进一步的分离分析。尽管如此，将转录组分析与预测的马铃薯 NBS-LRR 编码基因数据相结合，大大提高了结果的质量，并提供了相当数量的候选基因，为 S. phureja 提供了对马铃薯金色胞囊线虫的强抗性。
在抗性和敏感性基因型中进行组织特异性转录组的比较分析的组合，可以作为快速鉴定候选马铃薯 R 基因的方法，用于共分离分析，并可与基于基因组重测序的更复杂研究并行使用。
