Farrar W B, Sickle-Santanello B J, Keyhani-Rofagha S, DeCenzo J F, O'Toole R V
Department of Surgery, Ohio State University College of Medicine, Columbus.
Am J Surg. 1989 Apr;157(4):377-80. doi: 10.1016/0002-9610(89)90579-5.
Preliminary data from this institution suggested that flow cytometric DNA analysis was an objective prognostic indicator in archival localized squamous cell carcinoma of the tongue. Technical improvements were made, including analysis of tumor, normal tissue, and a combination of the two; standardized cursor placement; mathematic determination of tetraploid populations; and development of a statistical analysis. A larger number of patients (60) with this disease were reviewed. DNA content was related to disease-free survival, local recurrence, regional metastasis, and incidence of second primary tumors. There was no significant difference between aneuploid and diploid tumors with respect to the variables analyzed. We believe these technical improvements will enhance flow cytometric DNA analysis of paraffin-embedded tissues. However, in this retrospective review of localized squamous cell carcinoma of the tongue, DNA analysis was not a valuable prognostic indicator. Only prospective studies will address this issue.
该机构的初步数据表明，流式细胞术DNA分析是存档的局限性舌鳞状细胞癌的一个客观预后指标。进行了技术改进，包括对肿瘤、正常组织以及两者组合的分析；标准化游标放置；四倍体群体的数学测定；以及统计分析的开发。对更多数量（60例）患有这种疾病的患者进行了回顾。DNA含量与无病生存期、局部复发、区域转移以及第二原发性肿瘤的发生率相关。就所分析的变量而言，非整倍体肿瘤和二倍体肿瘤之间没有显著差异。我们认为这些技术改进将加强对石蜡包埋组织的流式细胞术DNA分析。然而，在这项对局限性舌鳞状细胞癌的回顾性研究中，DNA分析并不是一个有价值的预后指标。只有前瞻性研究才能解决这个问题。
