Passariello Claudio, Gigola Pierangelo, Testarelli Luca, Puttini Monica, Schippa Serena, Petti Stefano
Department of Public Health and Infectious Diseases, " Sapienza" University of Rome, Rome, Italy.
Department of Medical-Surgical Specialties, Radiologic and Public Health Sciences University of Brescia, Brescia, Italy.
Ann Stomatol (Roma). 2017 Nov 8;8(2):59-70. doi: 10.11138/ads/2017.8.2.071. eCollection 2017 Apr-Jun.
The present study aimed to investigate microbial patterns associated with disease progression and coinfection by different Herpesviruses in generalized aggressive periodontitis (GAP).
Microbiological samples were obtained from active (AS) and non-active (n-AS) sites in 165 subjects affected by GAP and were analyzed for 40 bacterial species by the Checkerboard DNA-DNA Hybridization technique and for Herpes simplex 1 (HSV-1), Human Cytomegalovirus (CMV), and Epstein Bar virus (EBV) by PCR.Common Factor Analysis and Multiple Regression Analysis were applied to disclose specific microbial patterns associated with the three viruses.
Herpesviruses were detected in 37.6% of subjects. Detection of each of the searched viruses was associated with specific patterns of subgingival biofilm in AS. Logistic regression analyses evidenced several virus/bacteria associations: i) EBV with ; ii) CMV with , and subsp; iii) HSV-1 with , , and .
Microbiological data suggest that Herpesviruses are probably not mere spectators of disease progression and that specific patterns of subgingival plaque are correlated with the presence of different Herpesviruses.
本研究旨在调查与广泛侵袭性牙周炎（GAP）中疾病进展及不同疱疹病毒合并感染相关的微生物模式。
从165例GAP患者的活动位点（AS）和非活动位点（n - AS）采集微生物样本，采用棋盘式DNA - DNA杂交技术分析40种细菌，通过聚合酶链反应分析单纯疱疹病毒1型（HSV - 1）、人巨细胞病毒（CMV）和爱泼斯坦 - 巴尔病毒（EBV）。应用共同因子分析和多元回归分析来揭示与这三种病毒相关的特定微生物模式。
37.6%的受试者检测到疱疹病毒。在活动位点，每种检测到的病毒都与龈下生物膜的特定模式相关。逻辑回归分析证实了几种病毒/细菌关联：i）EBV与 ；ii）CMV与 、 和 亚种；iii）HSV - 1与 、 、 和 。
微生物学数据表明，疱疹病毒可能不仅仅是疾病进展的旁观者，龈下菌斑的特定模式与不同疱疹病毒的存在相关。
