de León-Martínez L Díaz, Rodríguez-Aguilar M, Ocampo-Pérez R, Gutiérrez-Hernández J M, Díaz-Barriga F, Batres-Esquivel L, Flores-Ramírez R
Centro de Investigación Aplicada en Ambiente y Salud (CIAAS), Avenida Sierra Leona No. 550, CP 78210, Colonia Lomas Segunda Sección, San Luis Potosí, SLP, Mexico.
Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Avenida Sierra Leona No. 550, CP 78210, Colonia Lomas Segunda Sección, San Luis Potosí, SLP, Mexico.
Bull Environ Contam Toxicol. 2018 Mar;100(3):395-401. doi: 10.1007/s00128-017-2249-5. Epub 2018 Jan 3.
A molecularly imprinted polymer was developed and evaluated for selective determination of metronidazole (MNZ) in wastewater. This was achieved by using sodium methacrylate as monomer, toluene as porogen, ethylene glycol dimethacrylate as crosslinker, azobisisobutyronitrile as initiator and metronidazole as template molecule to generate the selectivity of the polymer for the compound, as well as non-imprinted polymers were synthesized. Two different polymerization approaches were used, bulk and emulsion and the polymers obtained by emulsion presented higher retention percentages the MIP 2-M presented the higher retention (83%). The performed method, was validated in fortified water, showing linearity from 10 up to 1000 ng/mL; limit of detection and quantification for compound were between 3 and 10 ng/mL, respectively. Finally, the method was applied in samples of a wastewater treatment plant in the city of San Luis Potosí, México, and the concentrations of MNZ in these samples were 84.1-114 ng/mL.
开发了一种分子印迹聚合物，并对其用于废水中甲硝唑（MNZ）的选择性测定进行了评估。这是通过使用甲基丙烯酸钠作为单体、甲苯作为致孔剂、乙二醇二甲基丙烯酸酯作为交联剂、偶氮二异丁腈作为引发剂以及甲硝唑作为模板分子来实现聚合物对该化合物的选择性，同时还合成了非印迹聚合物。使用了两种不同的聚合方法，本体聚合和乳液聚合，乳液聚合得到的聚合物呈现出更高的保留率，MIP 2-M的保留率最高（83%）。所采用的方法在加标水中进行了验证，线性范围为10至1000 ng/mL；该化合物的检测限和定量限分别在3至10 ng/mL之间。最后，该方法应用于墨西哥圣路易斯波托西市一家污水处理厂的样品，这些样品中MNZ的浓度为84.1 - 114 ng/mL。
