Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Taoyuan 33378, Taiwan.
Graduate Institute of Health Industry Technology, Research Center for Food and Cosmetic Safety, Chang Gung University of Science and Technology, Taoyuan 33303, Taiwan.
Int J Mol Sci. 2022 May 29;23(11):6104. doi: 10.3390/ijms23116104.
Sophoraflavanone G (SG), isolated from , has anti-inflammatory and anti-tumor bioactive properties. We previously showed that SG promotes apoptosis in human breast cancer cells and leukemia cells and reduces the inflammatory response in lipopolysaccharide-stimulated macrophages. We investigated whether SG attenuates airway hyper-responsiveness (AHR) and airway inflammation in asthmatic mice. We also assessed its effects on the anti-inflammatory response in human tracheal epithelial cells. Female BALB/c mice were sensitized with ovalbumin, and asthmatic mice were treated with SG by intraperitoneal injection. We also exposed human bronchial epithelial BEAS-2B cells to different concentrations of SG to evaluate its effects on inflammatory cytokine levels. SG treatment significantly reduced AHR, eosinophil infiltration, goblet cell hyperplasia, and airway inflammation in the lungs of asthmatic mice. In the lungs of ovalbumin-sensitized mice, SG significantly promoted superoxide dismutase and glutathione expression and attenuated malondialdehyde levels. SG also suppressed levels of Th2 cytokines and chemokines in lung and bronchoalveolar lavage samples. In addition, we confirmed that SG decreased pro-inflammatory cytokine, chemokine, and eotaxin expression in inflammatory BEAS-2B cells. Taken together, our data demonstrate that SG shows potential as an immunomodulator that can improve asthma symptoms by decreasing airway-inflammation-related oxidative stress.
槐黄酮 G（SG）是从 中分离出来的，具有抗炎和抗肿瘤的生物活性。我们之前的研究表明，SG 可促进人乳腺癌细胞和白血病细胞凋亡，并减轻脂多糖刺激的巨噬细胞中的炎症反应。我们研究了 SG 是否可减轻哮喘小鼠的气道高反应性（AHR）和气道炎症，并评估了其对人气管上皮细胞抗炎反应的影响。雌性 BALB/c 小鼠用卵清蛋白致敏，并用 SG 通过腹腔注射进行治疗。我们还将人支气管上皮 BEAS-2B 细胞暴露于不同浓度的 SG 以评估其对炎症细胞因子水平的影响。SG 治疗可显著降低哮喘小鼠的 AHR、嗜酸性粒细胞浸润、杯状细胞增生和气道炎症。在卵清蛋白致敏的小鼠肺部，SG 可显著促进超氧化物歧化酶和谷胱甘肽的表达并减轻丙二醛水平。SG 还可抑制肺和支气管肺泡灌洗液样本中的 Th2 细胞因子和趋化因子水平。此外，我们证实 SG 可降低炎症性 BEAS-2B 细胞中促炎细胞因子、趋化因子和嗜酸性粒细胞趋化因子的表达。总之，我们的数据表明，SG 作为一种免疫调节剂具有潜力，可通过降低与气道炎症相关的氧化应激来改善哮喘症状。
