刺阿干树果实水提取物改善链脲佐菌素诱导的糖尿病大鼠的糖尿病状况。
Aqueous Extract of Argania spinosa L. Fruits Ameliorates Diabetes in Streptozotocin-Induced Diabetic Rats.
作者信息
Hebi Morad, Khallouki Farid, Haidani A E L, Eddouks Mohamed
机构信息
Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, 52000, Errachidia, Morocco.
出版信息
Cardiovasc Hematol Agents Med Chem. 2018;16(1):56-65. doi: 10.2174/1871525716666180103163107.
INTRODUCTION
The argan tree (Argania spinosa L.) is an endemic species from south-western Morocco. Argan-based preparations have been widely used in Moroccan traditional medicine for their biological properties including diabetes especially argan oil. However, the antihypergycemic effect of the pericarp of A. spinosa fruit has never been evaluated.
OBJECTIVE
The purpose of this study was to investigate the effect of a single dose and daily oral administration for seven days of aqueous extract of pericarp of Argania spinosa fruit (A.E.P.F.A.S) in normal and streptozotocin (STZ) diabetic rats.
METHODS
The effect of A.E.P.F.A.S. (10 mg/kg) on blood glucose levels, plasma Total Cholesterol (TC) and triglyceride concentrations were measured in both normal and diabetic rats. The antioxidant capacity of the A.E.P.F.A.S was also demonstrated using test of DPPH (1-1-diphenyl 2-picryl hydrazyl). The histopathological changes in liver and pancreas have been evaluated in both normal and STZ diabetic rats. A preliminary phytochemical screening for various bioactive constituents and the antioxidant capacity were realized.
RESULTS
Single oral administration of A.E.P.F.A.S reduced blood glucose levels p 6 h after administration in STZ diabetic rats. Furthermore, blood glucose levels were decreased in STZ diabetic rats after seven days of treatment. According to the oral glucose tolerance test, the A.E.P.F.A.S. was shown to prevent significantly the increase in blood glucose levels in diabetic treated rats after glucose administration when compared to the control group. Moreover, A.E.P.F.A.S showed antioxidant activity and revealed the inhibitory concentration of 50% of free radicals (IC50) of 279.16 µg/ml.
CONCLUSION
This study demonstrates antihyperglycemic, lowering plasma cholesterol levels and antioxidant effects of A.E.P.F.A.S. in the severe diabetic state. Further investigations are needed to elucidate the mechanism( s) of action of A.E.P.F.A.S. and the active constituent(s) of the extract.
引言
阿甘树（Argania spinosa L.）是摩洛哥西南部的特有物种。基于阿甘树的制剂因其生物特性，尤其是阿甘油，在摩洛哥传统医学中被广泛使用。然而，阿甘树果实果皮的降血糖作用从未得到评估。
目的
本研究旨在探讨单剂量和连续7天每日口服阿甘树果实果皮水提取物（A.E.P.F.A.S）对正常和链脲佐菌素（STZ）诱导的糖尿病大鼠的影响。
方法
测定了A.E.P.F.A.S（10mg/kg）对正常和糖尿病大鼠血糖水平、血浆总胆固醇（TC）和甘油三酯浓度的影响。还通过1,1-二苯基-2-苦基肼（DPPH）试验证明了A.E.P.F.A.S的抗氧化能力。评估了正常和STZ糖尿病大鼠肝脏和胰腺的组织病理学变化。对各种生物活性成分进行了初步植物化学筛选并测定了抗氧化能力。
结果
单次口服A.E.P.F.A.S可使STZ糖尿病大鼠给药后6小时血糖水平降低。此外，治疗7天后STZ糖尿病大鼠的血糖水平下降。根据口服葡萄糖耐量试验，与对照组相比，A.E.P.F.A.S可显著预防糖尿病治疗大鼠给药后血糖水平的升高。此外，A.E.P.F.A.S表现出抗氧化活性，其对自由基的半数抑制浓度（IC50）为279.16μg/ml。
结论
本研究证明了A.E.P.F.A.S在重度糖尿病状态下具有降血糖、降低血浆胆固醇水平和抗氧化作用。需要进一步研究以阐明A.E.P.F.A.S的作用机制和提取物中的活性成分。
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