Hebi Morad, Farid Omra, Ajebli Mohammed, Eddouks Mohamed
Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, 52000, Errachidia, Morocco.
Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, 52000, Errachidia, Morocco.
Biomed Pharmacother. 2017 Mar;87:230-239. doi: 10.1016/j.biopha.2016.12.111. Epub 2017 Jan 3.
The purpose of this study was to investigate the effect of a single dose and daily oral administration for seven days of the aerial part aqueous extract (A.P.A.E) of Tamarix articulata Vahl. (T. articulata) (5mg/kg) on blood glucose levels in both normal and streptozotocin-induced diabetic rats (STZ). Single oral administration of T. articulata A.P.A.E reduced blood glucose levels 6h after administration in normal rats (p<0.0001) and STZ diabetic rats (p<0.001). Furthermore, blood glucose levels were decreased in both normal (p<0.05) and STZ diabetic rats (p<0.0001) after seven days of treatment. Moreover, no significant changes in body weight in normal and STZ rats were shown. According to the oral glucose tolerance test, the aqueous extract of T. articulata (5mg/kg) was shown to prevent significantly the increase on blood glucose levels in both normal and diabetic treated rats 30min, 60min and 120min after glucose administration when compared to the control group. Additionally, histopathological analysis revealed the beneficial effect of T. articulata on pancreas and liver. Finally, the antioxydant activity of the aqueous extract of Tamarix articulata was evaluated by the method of trapping of free radical 2,2-diphenyl-1 picrylhydrazyl (DPPH). Tamarix articulata revealed inhibitory concentrations of 50% of free radicals (IC50) of 203.15μg/ml. In contrast, the synthetic antioxidant butylhydroxytoluene (BHT) has showed an IC50 equal to 13.71μg/ml. In conclusion, this study demonstrates antihyperglycemic, hypoglycemic and antioxidant effects of T. articulata in severe diabetic state thus warrants further investigation on its major compounds as well as mechanistic studies.
本研究旨在探讨单剂量及连续7天每日口服关节柽柳地上部分水提取物（A.P.A.E）（5mg/kg）对正常大鼠和链脲佐菌素诱导的糖尿病大鼠（STZ）血糖水平的影响。正常大鼠和STZ糖尿病大鼠单次口服关节柽柳A.P.A.E后6小时血糖水平降低（正常大鼠p<0.0001，STZ糖尿病大鼠p<0.001）。此外，治疗7天后，正常大鼠（p<0.05）和STZ糖尿病大鼠（p<0.0001）的血糖水平均降低。而且，正常大鼠和STZ大鼠的体重均无显著变化。根据口服葡萄糖耐量试验，与对照组相比，关节柽柳水提取物（5mg/kg）在正常和糖尿病治疗大鼠中均能显著预防葡萄糖给药后30分钟、60分钟和120分钟血糖水平的升高。此外，组织病理学分析显示关节柽柳对胰腺和肝脏具有有益作用。最后，采用2,2-二苯基-1-苦基肼自由基（DPPH）捕获法评估关节柽柳水提取物的抗氧化活性。关节柽柳的自由基半数抑制浓度（IC50）为203.15μg/ml。相比之下，合成抗氧化剂丁基羟基甲苯（BHT）的IC50为13.71μg/ml。总之，本研究证明了关节柽柳在严重糖尿病状态下具有降血糖、低血糖和抗氧化作用，因此有必要对其主要化合物进行进一步研究以及开展作用机制研究。
