Ajebli Mohammed, Eddouks Mohamed
Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 509, Boutalamine, Errachidia, 52000, Morocco.
Cardiovasc Hematol Disord Drug Targets. 2017;17(2):142-152. doi: 10.2174/1871529X17666170918140817.
Buxus sempervirens L. (Boxwood) is a medicinal plant used in Moroccan traditional medicine for diabetes treatment in Morocco. However; there is no experimental support of this ethnopharmacological use.
The present investigation is aimed at studying the antidiabetic and the preliminary phytochemical of the aqueous extract of the leaves of Buxus sempervirens (AELBS). AELBS (5 mg/kg) was administered by gavage to normal and streptozotocin-induced diabetic rats and blood glucose levels were measured for 6 hours (acute study) and 15 days (chronic study). Oral glucose test tolerance (OGTT) and histopathological examination of liver and pancreas were carried out using standard methods. Furthermore, preliminary phytochemical screening and quantification of phenolic and flavonoid contents were also realized.
AELBS reduced the blood glucose of both healthy and diabetic rats. This extract was also able to improve oral glucose tolerance in diabetic rats and it ameliorated hepatic histology, however, no enhancement was noticed in pancreatic histology. Preliminary phytochemical study revealed that AELBS possesses many chemical compounds such as alkaloids, phenolic compounds (flavonoids, tannins, and cyanidins), carbohydrates, reducing sugars and mucilage. Additionally, this extract is rich in phenolic compounds including flavonoids.
According to all these findings, we conclude that AELBS at a low dose demonstrated a potent antihyperglycemic effect in streptozotocin rats and further investigations should be necessary for supporting the use of this plant in the management of diabetes by the Moroccan population.
欧洲黄杨是一种用于摩洛哥传统医学治疗糖尿病的药用植物。然而，这种民族药理学用途尚无实验依据。
本研究旨在探讨欧洲黄杨叶水提取物（AELBS）的抗糖尿病作用及其初步的植物化学性质。将AELBS（5毫克/千克）通过灌胃给予正常大鼠和链脲佐菌素诱导的糖尿病大鼠，并在6小时（急性研究）和15天（慢性研究）内测量血糖水平。采用标准方法进行口服葡萄糖耐量试验（OGTT）以及肝脏和胰腺的组织病理学检查。此外，还进行了初步的植物化学筛选以及酚类和黄酮类成分的定量分析。
AELBS降低了健康大鼠和糖尿病大鼠的血糖。该提取物还能够改善糖尿病大鼠的口服葡萄糖耐量，并改善肝脏组织学，但胰腺组织学未见改善。初步的植物化学研究表明，AELBS含有多种化合物，如生物碱、酚类化合物（黄酮类、单宁和花青素）、碳水化合物、还原糖和黏液质。此外，该提取物富含包括黄酮类在内的酚类化合物。
根据所有这些发现，我们得出结论，低剂量的AELBS在链脲佐菌素诱导的大鼠中显示出强大的降血糖作用，有必要进行进一步研究以支持摩洛哥人群将这种植物用于糖尿病管理。
