Team of Ethnopharmacology and Pharmacognosy, Department of Biology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University of Meknes, BP 509, Boutalamine, 52000 Errachidia, Morocco.
Endocr Metab Immune Disord Drug Targets. 2020;20(9):1504-1513. doi: 10.2174/1871530320666200513081312.
The current investigation aimed to assess the antioxidant, antidiabetic and antilipidemic effects of the aqueous extract of aerial part of Cotula cinerea (C. cinerea).
Cotula cinerea (Del). which belongs to the Asteraceae family is commonly used traditionally for the treatment of diabetes.
The objective of the study was to study the effect of the aqueous C. cinerea extract on glucose and lipid metabolism in normal and streptozotocin-induced diabetic rats using a single and repeated oral administration.
A preliminary phytochemical screening and the quantification of phenolic and flavonoid contents as well as the antioxidant activity using three methods (DPPH, FRAP and ABTS) were carried out. The effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of aerial part of Cotula cinerea (AEAPCC) at a dose of 20 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of the pancreas and liver was carried out according to the Hematoxylin-Eosin method.
AEAPCC (20 mg/kg) showed a significant blood glucose-lowering activity in both normal and diabetic rats after a single and repeated oral administration during 15 days. The aqueous extract was also able to decrease the plasma triglycerides levels in both normal and diabetic rats after 15 days of oral treatment at a dose of 20 mg/Kg while no effect was observed on plasma cholesterol levels. In addition, the results show that AEAPCC exhibits an in vitro antioxidant activity using different tests. Histopathological analysis of the pancreas and liver of AEAPCC-treated diabetic rats has revealed that AEAPCC had a beneficial effect on the architecture of these organs while no improvement of glucose tolerance was noticed using the glucose tolerance test. Furthermore, the results showed that the extract is rich in several phytochemical compounds and exhibited an important antioxidant activity. The phytochemical screening revealed that AEAPCC contains polyphenolic compounds, flavonoids, tannins, alkaloids, saponins, quinones, sterols, terpenoids, anthroquinones and reducing sugars. Whereas, it is free from glycosides.
In conclusion, this study demonstrates that Cotula cinerea possesses a beneficial effect on diabetes. Further investigations are required to study the mechanism of action of the antidiabetic effect of this plant.
本研究旨在评估 Cotula cinerea（C. cinerea）地上部分水提物的抗氧化、抗糖尿病和抗脂质作用。
Cotula cinerea（Del）属于菊科，传统上常用于治疗糖尿病。
研究目的是使用单次和重复口服给药的方法，研究 Cotula cinerea 水提物对正常和链脲佐菌素诱导的糖尿病大鼠葡萄糖和脂质代谢的影响。
进行了初步的植物化学筛选，并通过三种方法（DPPH、FRAP 和 ABTS）定量测定了酚类和类黄酮含量以及抗氧化活性。在正常和链脲佐菌素诱导的糖尿病大鼠中，检查了 Cotula cinerea 地上部分水提物（AEAPCC）在 20mg/kg 剂量下单次和重复（15 天治疗）口服给药对葡萄糖和脂质谱的影响。此外，根据苏木精-伊红法对胰腺和肝脏进行了组织病理学检查。
AEAPCC（20mg/kg）在单次和重复口服给药 15 天内均显示出显著的降血糖活性，无论是在正常大鼠还是糖尿病大鼠中。水提物还能够降低正常和糖尿病大鼠在 15 天口服治疗 20mg/Kg 时的血浆三酰甘油水平，而对血浆胆固醇水平没有影响。此外，结果表明 AEAPCC 在不同测试中表现出体外抗氧化活性。AEAPCC 治疗糖尿病大鼠的胰腺和肝脏组织病理学分析表明，AEAPCC 对这些器官的结构具有有益作用，而葡萄糖耐量试验未观察到葡萄糖耐量的改善。此外，结果表明提取物富含多种植物化学化合物，并表现出重要的抗氧化活性。植物化学筛选表明，AEAPCC 含有多酚类化合物、类黄酮、单宁、生物碱、皂苷、醌类、甾醇、萜类、蒽醌和还原糖。而它不含糖苷。
总之，本研究表明 Cotula cinerea 对糖尿病具有有益作用。需要进一步研究以研究这种植物的抗糖尿病作用的作用机制。
