自交能育线虫的快速基因组收缩揭示了精子竞争蛋白。
Rapid genome shrinkage in a self-fertile nematode reveals sperm competition proteins.
作者信息
Yin Da, Schwarz Erich M, Thomas Cristel G, Felde Rebecca L, Korf Ian F, Cutter Asher D, Schartner Caitlin M, Ralston Edward J, Meyer Barbara J, Haag Eric S
机构信息
Department of Biology, University of Maryland, College Park, MD 20742, USA.
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
出版信息
Science. 2018 Jan 5;359(6371):55-61. doi: 10.1126/science.aao0827.
Abstract
To reveal impacts of sexual mode on genome content, we compared chromosome-scale assemblies of the outcrossing nematode to its self-fertile sibling species, 's genome resembles that of outcrossing relatives but encodes 31% more protein-coding genes than genes lacking orthologs were disproportionately small and male-biased in expression. These include the () gene family, which encodes sperm surface glycoproteins conserved only in outcrossing species. Sperm from -null males of outcrossing failed to compete with wild-type sperm, despite normal fertility in noncompetitive mating. Restoring to males was sufficient to enhance sperm competitiveness. Thus, sex has a pervasive influence on genome content that can be used to identify sperm competition factors.
摘要
为揭示有性生殖方式对基因组内容的影响，我们将异交线虫的染色体水平组装与其自交能育的姊妹物种进行了比较，[物种名称]的基因组类似于异交亲属的基因组，但编码的蛋白质编码基因比[物种名称]多31%。缺乏直系同源物的基因不成比例地小且在表达上偏向雄性。这些基因包括[基因名称]基因家族，该家族编码仅在异交物种中保守的精子表面糖蛋白。异交[物种名称]的[基因名称]缺失雄性的精子尽管在非竞争性交配中具有正常生育力，但无法与野生型精子竞争。将[基因名称]恢复到[物种名称]雄性足以增强精子竞争力。因此，性别对基因组内容有广泛影响，可用于识别精子竞争因子。
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