Zhang Lili, Zhao Wei, Zhao Chunshan, Jin Hong, Wang Bin, Wang Bangmao
Department of Digestive Diseases, General Hospital, Tianjin Medical University, Tianjin, China.
Physiol Res. 2018 May 4;67(2):275-282. doi: 10.33549/physiolres.933652. Epub 2018 Jan 5.
Electric stimulation (ES) could induce contraction of intestinal smooth muscle. The aim of this study was to analyze the effects of ES on esophageal motility and the underlying mechanism in vivo. Twenty-eight rabbits were equipped with a pair of subserosa electrodes (connected to an electrical stimulator) in the lower segment of the esophagus. The ES signal consisted of bipolar rectangular pulse trains, lasting for 3 s, with different amplitudes (1 mA, 3 mA, 5 mA and 10 mA), and frequencies (10 Hz, 20 Hz and 50 Hz). The amplitude of the contraction was recognized by high-resolution manometry. The effect of ES was tested under anesthesia and following administration of atropine, phentolamine or L-NAME. ES induced esophageal contraction at the stimulated site. A statistically significant increase in esophageal pressure was observed when the stimulation amplitude was above 3 mA. The increase in esophageal pressure was associated with the amplitude of stimulus as well as the frequency. During stimulation, atropine, phentolamine and L-NAME had no effect on the increase of esophageal pressure induced by ES. These findings implied that ES induced esophageal contraction were not mediated via the NANC, adrenergic or cholinergic pathway. The amplitude of esophageal contraction was current and frequency dependent.
电刺激（ES）可诱导肠道平滑肌收缩。本研究旨在分析电刺激对体内食管动力的影响及其潜在机制。28只家兔在食管下段安装了一对浆膜下电极（连接至电刺激器）。电刺激信号由双极矩形脉冲序列组成，持续3秒，具有不同的幅度（1毫安、3毫安、5毫安和10毫安）和频率（10赫兹、20赫兹和50赫兹）。收缩幅度通过高分辨率测压法识别。在麻醉状态下以及给予阿托品、酚妥拉明或L-精氨酸甲酯（L-NAME）后测试电刺激的效果。电刺激在刺激部位诱导食管收缩。当刺激幅度高于3毫安时，观察到食管压力有统计学意义的增加。食管压力的增加与刺激幅度以及频率有关。在刺激过程中，阿托品、酚妥拉明和L-NAME对电刺激诱导的食管压力增加没有影响。这些发现表明，电刺激诱导的食管收缩不是通过非肾上腺素能非胆碱能（NANC）、肾上腺素能或胆碱能途径介导的。食管收缩幅度取决于电流和频率。
