1National Marine Biodiversity Institute of Korea, 101-75, Jangsan-ro, Janghang-eup, Seocheon-gun Chungcheongnam-do 33662, Republic of Korea.
2Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology, 385, Haeyang-ro, Yeongdo-gu, Busan, 49111, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Dec;68(12):3886-3891. doi: 10.1099/ijsem.0.003081. Epub 2018 Oct 24.
A yellow-pigmented bacterium with the ability to degrade starch, designated MEBiC07310, was isolated from tidal flat sediment collected in Taean County, Republic of Korea. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain MEBiC07310 was affiliated with the genus Flavobacterium in the phylum Bacteroidetes and showed that the strain was most closely related to Flavobacterium haoranii LQY-7 (96.8 % similarity), followed by Flavobacterium indicum GPTSA 100-9 (95.2 %) and Flavobacterium urocaniciphilum YIT 12746 (94.6 %). Genome-based analysis of the average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) of strain MEBiC07310 compared with F. haoranii LQY-7 and F. indicum GPTSA 100-9 yielded ANI values of 77.0 and 73.3 % and DDH values of 18.0±2.7 and 16.1±3.6 %, respectively. The DNA G+C content of strain MEBiC07310 was 35.2 mol%. Cells of the strain were aerobic, Gram-stain-negative and rod-shaped, and negative for flexirubin-type pigments. Growth was observed at 17-43 °C (optimum 32 °C), at pH 5.0-8.0 (optimum pH 7.0) and with 0-3 % (w/v) NaCl (optimum 1 %). The major fatty acids (>10 %) of strain MEBiC07310 were iso-C15 : 0, iso-C17 : 0 3-OH, summed feature 1 (iso-C15 : 1 H and/or C13 : 0 3-OH) and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The major respiratory quinone was menaquinone MK-6. Based on its phenotypic and genotypic characteristics, strain MEBiC07310 should be classified as representing a novel species of the genus Flavobacterium, for which the name Flavobacterium sediminis sp. nov. is proposed. The type strain is MEBiC07310 (=KCTC 62132=JCM 32291).
一株能够降解淀粉的黄色着色细菌，被命名为 MEBiC07310，从韩国泰安郡的潮汐滩沉积物中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MEBiC07310属于黄杆菌属，在拟杆菌门中，与哈氏黄杆菌 LQY-7（96.8%相似度）最为密切相关，其次是印度黄杆菌 GPTSA 100-9（95.2%相似度）和尿囊酸好氧菌 YIT 12746（94.6%相似度）。基于平均核苷酸同一性（ANI）和菌株 MEBiC07310 的 DNA-DNA 杂交（DDH）的基因组分析与哈氏黄杆菌 LQY-7 和印度黄杆菌 GPTSA 100-9 相比，ANI 值分别为 77.0%和 73.3%，DDH 值分别为 18.0±2.7%和 16.1±3.6%。菌株 MEBiC07310 的 DNA G+C 含量为 35.2mol%。该菌株为需氧、革兰氏阴性、杆状，不产生弹性红质型色素。在 17-43°C（最佳 32°C）、pH5.0-8.0（最佳 pH7.0）和 0-3%（w/v）NaCl（最佳 1%）下生长。菌株 MEBiC07310 的主要脂肪酸（>10%）为 iso-C15:0、iso-C17:0 3-OH、summed feature 1（iso-C15:1H 和/或 C13:0 3-OH）和 summed feature 3（C16:1ω6c 和/或 C16:1ω7c）。主要呼吸醌为menaquinone MK-6。基于其表型和基因型特征，菌株 MEBiC07310 应归类为代表黄杆菌属的一个新种，现提议将其命名为 Flavobacterium sediminis sp. nov.。模式菌株为 MEBiC07310（=KCTC 62132=JCM 32291）。
