Center of Basic Research, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece.
Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece.
Int J Mol Sci. 2018 Jan 5;19(1):158. doi: 10.3390/ijms19010158.
Dental stem cells (DSCs) have emerged as a promising tool for basic research and clinical practice. A variety of adult stem cell (ASC) populations can be isolated from different areas within the dental tissue, which, due to their cellular and molecular characteristics, could give rise to different outcomes when used in potential applications. In this study, we performed a high-throughput molecular comparison of two primary human adult dental stem cell (hADSC) sub-populations: Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs) and Periodontal Ligament Stem Cells (PDLSCs). A detailed proteomic mapping of SHEDs and PDLSCs, via employment of nano-LC tandem-mass spectrometry (MS/MS) revealed 2032 identified proteins in SHEDs and 3235 in PDLSCs. In total, 1516 proteins were expressed in both populations, while 517 were unique for SHEDs and 1721 were exclusively expressed in PDLSCs. Further analysis of the recorded proteins suggested that SHEDs predominantly expressed molecules that are involved in organizing the cytoskeletal network, cellular migration and adhesion, whereas PDLSCs are highly energy-producing cells, vastly expressing proteins that are implicated in various aspects of cell metabolism and proliferation. Applying the Rho-GDI signaling pathway as a paradigm, we propose potential biomarkers for SHEDs and for PDLSCs, reflecting their unique features, properties and engaged molecular pathways.
牙髓干细胞（DSCs）已成为基础研究和临床实践的一种有前途的工具。多种成体干细胞（ASC）群体可以从牙齿组织的不同区域中分离出来，由于其细胞和分子特征，当用于潜在应用时可能会产生不同的结果。在这项研究中，我们对两种主要的人成体牙髓干细胞（hADSC）亚群：人脱落乳牙牙髓干细胞（SHEDs）和牙周韧带干细胞（PDLSCs）进行了高通量分子比较。通过使用纳升液相串联质谱（MS/MS）对 SHEDs 和 PDLSCs 进行详细的蛋白质组学图谱分析，在 SHEDs 中鉴定出 2032 种蛋白，在 PDLSCs 中鉴定出 3235 种蛋白。总共有 1516 种蛋白在两种群体中表达，而 517 种蛋白在 SHEDs 中是独特的，1721 种蛋白仅在 PDLSCs 中表达。对记录蛋白的进一步分析表明，SHEDs 主要表达参与细胞骨架网络组织、细胞迁移和黏附的分子，而 PDLSCs 是高能量产生细胞，广泛表达参与细胞代谢和增殖各个方面的蛋白。我们应用 Rho-GDI 信号通路作为范例，提出了 SHEDs 和 PDLSCs 的潜在生物标志物，反映了它们的独特特征、性质和参与的分子途径。
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