水凝胶中添加人血小板裂解液增强间充质干细胞的多向分化能力。

Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells.

机构信息

School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 112 Lab, Lihua BLDG, No.30 Xueyuan Road, Haidian District, Beijing, 100083, China.

Daxing Research Institute, University of Science and Technology Beijing, Beijing, 100083, China.

出版信息

J Nanobiotechnology. 2022 Apr 2;20(1):176. doi: 10.1186/s12951-022-01387-9.

Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients will increase the risk of zoonotic disease transmission. Human platelet lysate (HPL) is a potential surrogate and used in human cell expansion with reliability in clinical applications. In this study, we synthesized chitosan/gelatin/gellan gum hydrogel supplemented with HPL and investigated the effect of 3D culture for SHED. TMT-tagged proteomics was used to decipher the secretome protein profiles of SHEDs and a total of 3209 proteins were identified, of which 23 were up-regulated and 192 were down-regulated. The results showed that hydrogel supplemented with HPL promoted SHED proliferation. After induction, the hydrogel coating contributed to osteogenic differentiation, adipogenic differentiation and differentiation into neural-like cells of SHED. SHED encapsulated in a hydrogel promotes migration and angiogenesis of HUVEC. In conclusion, our research found that hydrogel supplemented with HPL can be used as a method for SHED in standardized production and can contribute to the clinical application of SHED in cell therapy.

人脱落乳牙来源的干细胞（SHED）可用作一种潜在的临床材料。但是使用异种成分会增加人畜共患病传播的风险。人血小板裂解液（HPL）是一种潜在的替代品，可用于人类细胞扩增，在临床应用中具有可靠性。在这项研究中，我们合成了壳聚糖/明胶/结冷胶水凝胶，补充了 HPL，并研究了 3D 培养对 SHED 的影响。TMT 标记蛋白质组学用于破译 SHED 的分泌蛋白谱，共鉴定出 3209 种蛋白质，其中 23 种上调，192 种下调。结果表明，补充 HPL 的水凝胶促进了 SHED 的增殖。诱导后，水凝胶涂层有助于 SHED 的成骨分化、脂肪分化和向神经样细胞分化。包封在水凝胶中的 SHED 促进 HUVEC 的迁移和血管生成。总之，我们的研究发现，补充 HPL 的水凝胶可用作 SHED 的标准化生产方法，并有助于 SHED 在细胞治疗中的临床应用。