Hosmani Jagadish, Assiri Khalil, Almubarak Hussain Mohammed, Mannakandath Master Luqman, Al-Hakami Ahmed, Patil Shankargouda, Babji Deepa, Sarode Sachin, Devaraj Anantharam, Chandramoorthy Harish C
Diagnostic Dental Sciences, College of Dentistry, King Khalid University, Abha 61471, Asir, Saudi Arabia.
Diagnostic Dental Sciences, King Khalid University, Abha 61471, Asir, Saudi Arabia.
World J Stem Cells. 2020 Oct 26;12(10):1214-1236. doi: 10.4252/wjsc.v12.i10.1214.
The proteomic signature or profile best describes the functional component of a cell during its routine metabolic and survival activities. Additional complexity in differentiation and maturation is observed in stem/progenitor cells. The role of functional proteins at the cellular level has long been attributed to anatomical niches, and stem cells do not deflect from this attribution. Human dental stem cells (hDSCs), on the whole, are a combination of mesenchymal and epithelial coordinates observed throughout craniofacial bones to pulp.
To specify the proteomic profile and compare each type of hDSC with other mesenchymal stem cells (MSCs) of various niches. Furthermore, we analyzed the characteristics of the microenvironment and preconditioning changes associated with the proteomic profile of hDSCs and their influence on committed lineage differentiation.
Literature searches were performed in PubMed, EMBASE, Scopus, and Web of Science databases, from January 1990 to December 2018. An extra inquiry of the grey literature was completed on Google Scholar, ProQuest, and OpenGrey. Relevant MeSH terms (PubMed) and keywords related to dental stem cells were used independently and in combination.
The initial search resulted in 134 articles. Of the 134 full-texts assessed, 96 articles were excluded and 38 articles that met the eligibility criteria were reviewed. The overall assessment of hDSCs and other MSCs suggests that differences in the proteomic profile can be due to stem cellular complexity acquired from varied tissue sources during embryonic development. However, our comparison of the proteomic profile suffered inconsistencies due to the heterogeneity of various hDSCs. We believe that the existence of a heterogeneous population of stem cells at a given niche determines the modalities of regeneration or tissue repair. Added prominences to the differences present between various hDSCs have been reasoned out.
Systematic review on proteomic studies of various hDSCs are promising as an eye-opener for revisiting the proteomic profile and in-depth analysis to elucidate more refined mechanisms of hDSC functionalities.
蛋白质组特征或图谱最能描述细胞在其日常代谢和存活活动中的功能成分。在干/祖细胞中观察到分化和成熟过程存在额外的复杂性。功能蛋白在细胞水平上的作用长期以来一直归因于解剖学微环境，干细胞也不例外。总体而言，人牙干细胞（hDSC）是在整个颅面骨到牙髓中观察到的间充质和上皮坐标的组合。
明确hDSC的蛋白质组图谱，并将每种类型的hDSC与不同微环境的其他间充质干细胞（MSC）进行比较。此外，我们分析了与hDSC蛋白质组图谱相关的微环境特征和预处理变化及其对定向谱系分化的影响。
于1990年1月至2018年12月在PubMed、EMBASE、Scopus和Web of Science数据库中进行文献检索。在Google Scholar、ProQuest和OpenGrey上完成了对灰色文献的额外查询。独立并组合使用与牙干细胞相关的相关医学主题词（PubMed）和关键词。
初步检索得到134篇文章。在评估的134篇全文中，排除了96篇文章，对符合纳入标准的38篇文章进行了综述。对hDSC和其他MSC的总体评估表明，蛋白质组图谱的差异可能归因于胚胎发育过程中从不同组织来源获得的干细胞复杂性。然而，由于各种hDSC的异质性，我们对蛋白质组图谱的比较存在不一致性。我们认为，给定微环境中干细胞异质性群体的存在决定了再生或组织修复的方式。已经阐明了各种hDSC之间存在差异的其他突出原因。
对各种hDSC的蛋白质组学研究进行系统综述有望成为重新审视蛋白质组图谱和深入分析以阐明hDSC功能更精细机制的契机。
