Losada-Pérez Patricia, Polat Ozge, Parikh Atul N, Seker Erkin, Renner Frank Uwe
Institute for Materials Research IMO, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium and IMEC vzw, division IMOMEC, Wetenschapspark 1, B-3590 Diepenbeek, Belgium.
Department of Chemical Engineering, University of California-Davis, Davis, California 95616.
Biointerphases. 2018 Jan 5;13(1):011002. doi: 10.1116/1.5010249.
Nanoporous gold (np-Au) is a nanostructured metal with many desirable attributes. Despite the growing number of applications of nanoporous materials, there are still open questions regarding their fabrication and subsequent surface functionalization. For example, the hydrophobic nature of gold surfaces makes the formation of planar supported lipid layers challenging. Here, the authors engineer the interface between np-Au and 1,2-dioleoyl-sn-glycero-3-phosphocholine lipid layers using well-differentiated approaches based on vesicle adsorption and solvent exchange methods. The results reveal that the nanotopography of the np-Au surface plays a clear role in the vesicle adsorption process. Compared to vesicle adsorption, the solvent exchange method proves successful in the formation of planar supported lipid bilayers in both np-Au and planar Au surfaces, being less sensitive to the surface morphological features. The influence of nanostructured surfaces on lipid layer formation is determined by the driving mechanisms behind each process, i.e., the balance of adhesion and cohesion forces in vesicle adsorption and lyotropic lipid phase transitions in solvent exchange, respectively. A better understanding of such interactions will contribute to the development of a variety of applications, from electrochemical biosensors to drug screening and delivery systems, using nanoporous gold coated with stimuli-responsive lipid layers.
纳米多孔金（np-Au）是一种具有许多理想特性的纳米结构金属。尽管纳米多孔材料的应用越来越多，但关于其制备及后续表面功能化仍存在一些悬而未决的问题。例如，金表面的疏水性使得平面支撑脂质层的形成具有挑战性。在此，作者基于囊泡吸附和溶剂交换方法，采用精心设计的方法来构建np-Au与1,2-二油酰基-sn-甘油-3-磷酸胆碱脂质层之间的界面。结果表明，np-Au表面的纳米拓扑结构在囊泡吸附过程中起着明显作用。与囊泡吸附相比，溶剂交换方法在np-Au和平面金表面形成平面支撑脂质双层方面均取得成功，且对表面形态特征的敏感度较低。纳米结构表面对脂质层形成的影响取决于每个过程背后的驱动机制，即分别取决于囊泡吸附中粘附力和内聚力的平衡以及溶剂交换中溶致液晶脂质相转变。更好地理解此类相互作用将有助于开发各种应用，从电化学生物传感器到使用涂覆有刺激响应脂质层的纳米多孔金的药物筛选和递送系统。
