Coleman R, Rahman K, Kan K S, Parslow R A
Department of Biochemistry, University of Birmingham, U.K.
Biochem J. 1989 Feb 15;258(1):17-22. doi: 10.1042/bj2580017.
The control of biliary phospholipid and cholesterol secretions by bile acid was studied by using the technique of retrograde intrabiliary injection. Taurocholate (TC), a moderately hydrophobic bile acid, taurodehydrocholate (TDHC), a hydrophilic non-micelle-forming bile acid, and 3-[(3-cholamidopropyl)-dimethylammonio]propane-1-sulphonate (CHAPS), a detergent, were individually administered by retrograde intrabiliary injection (RII) into the biliary tree, and bile acids, phospholipids and cholesterol subsequently appearing in the bile were measured. TC (1.3 mumol; 45 microliters) injected retrogradely provoked a 3.5-fold increase in biliary phospholipid output for 40 min, as compared with the saline control. Injection of 2.7 mumol of TC (90 microliters) caused a 7.5-fold increase in phospholipid output, which reached a peak at 12 min after RII, and phospholipid output continued for 40 min. Cholesterol output was also elicited under these conditions, showing both dose-dependency and extended secretion. Injection of 1.8 mumol of TDHC caused very little increase in either biliary phospholipid or cholesterol. Injection of 0.9 mumol of CHAPS (45 microliters) provoked a single substantial peak of phospholipid output in the 3 min bile sample. T.l.c. analysis of the phospholipid extracts of the bile collected after each compound showed, for TC, a single compound which co-migrated with the phosphatidylcholine standard, whereas for CHAPS substantial amounts of other phospholipids were present.
采用逆行胆管内注射技术研究了胆汁酸对胆汁中磷脂和胆固醇分泌的控制作用。将牛磺胆酸盐（TC），一种中度疏水性胆汁酸；牛磺去氢胆酸盐（TDHC），一种亲水性非胶束形成胆汁酸；以及3-[(3-胆酰胺丙基)-二甲基铵]丙烷-1-磺酸盐（CHAPS），一种去污剂，分别通过逆行胆管内注射（RII）注入胆管树，随后测量胆汁中出现的胆汁酸、磷脂和胆固醇。与生理盐水对照相比，逆行注射1.3 μmol（45微升）的TC在40分钟内使胆汁磷脂输出增加了3.5倍。注射2.7 μmol的TC（90微升）使磷脂输出增加了7.5倍，在RII后12分钟达到峰值，磷脂输出持续40分钟。在这些条件下也引发了胆固醇输出，显示出剂量依赖性和延长分泌。注射1.8 μmol的TDHC对胆汁磷脂或胆固醇的增加非常小。注射0.9 μmol的CHAPS（45微升）在3分钟胆汁样本中引发了一个单一的磷脂输出高峰。对每种化合物注射后收集的胆汁磷脂提取物进行薄层层析分析表明，对于TC，有一种与磷脂酰胆碱标准品共迁移的单一化合物，而对于CHAPS，则存在大量其他磷脂。
