College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China.
Int J Mol Sci. 2018 Aug 30;19(9):2579. doi: 10.3390/ijms19092579.
Low temperature stress adversely affects plant growth and development. Isolation and characterization of cold response genes from cold-tolerant plants help to understand the mechanism underlying low temperature tolerance. In this study, , a cold-regulated (COR) gene isolated from , was transferred to plants to investigate its function. Real-time quantitative PCR analysis revealed that expression was induced by cold. Subcellular localization revealed that the PsCor413pm2-green fluorescent protein (GFP) fusion protein localized to the plasma membrane in tobacco and plants. Furthermore, overexpression of in plants enhanced tolerance to low temperature stress. Transgenic roots had more influx of Ca after a cold shock than wild-type plants, as shown using non-invasive micro-test technology (NMT). Moreover, the transcription abundance of five and two C-repeat (CRT) binding factor (CBF) genes in transgenic plants was higher than that in the wild-type plants under cold stress. Taken together, our results suggest that overexpression of enhances low temperature tolerance in plants by affecting Ca flux and the expression of stress-related and genes.
低温胁迫会对植物的生长和发育产生不利影响。从耐冷植物中分离和鉴定冷响应基因有助于理解低温耐受性的机制。在这项研究中，从 中分离到一个冷调节（COR）基因 ，并将其转入 植物中以研究其功能。实时定量 PCR 分析显示 表达受低温诱导。亚细胞定位显示 PsCor413pm2-绿色荧光蛋白（GFP）融合蛋白在烟草和 植物中定位于质膜。此外，在 植物中超表达 增强了对低温胁迫的耐受性。用非侵入性微测技术（NMT）显示，冷冲击后，转基因 根中 Ca 的内流比野生型植物多。此外，在冷胁迫下，转基因 植物中五个 和两个 C-重复（CRT）结合因子（CBF）基因的转录丰度高于野生型植物。综上所述，我们的结果表明，过表达 通过影响 Ca 流和与应激相关的 和 基因的表达来增强 植物的低温耐受性。
