Mechanical Engineering Department, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil.
Mechanical Engineering Department, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil; Materials Engineering Special Coordination, Federal University of Santa Catarina, Blumenau, SC 89036-002, Brazil.
Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110849. doi: 10.1016/j.msec.2020.110849. Epub 2020 Mar 19.
Membranes for guided bone regeneration represent valuable resources, preventing fibroblast infiltration and aiding anatomical bone reconstruction. Nonetheless, available membranes lack bone regenerative capacity, suitable mechanical behavior, or adequate degradation profile. Therefore, to overcome these limitations, this study developed bilayer membranes with a dense layer (dry phase inversion) of PLGA (poly(lactic-co-glycolic acid)):HAp (hydroxyapatite) - 95:05 (wt%) - and an electrospun layer of PLGA and HAp:β-TCP (β-tricalcium phosphate) with ratios of 60:40, 70:30 and 85:15 (wt%), evaluating its mechanical, morphological and in vitro properties. The bilayer membranes displayed adequate interlayer adhesion, dense layer pore size of 4.20 μm and electrospun layer with porosity degree of 38.2%, thus capable of preventing fibroblast infiltration while allowing osteoblast migration and nutrient permeation. They also showed T of 82 °C and higher storage modulus, which was constant up to 54.6 °C, characteristics important for membrane implantation and use with no mechanical compromise. In vitro degradation mass loss was only 10% after 60 days, a profile suitable for the application requirement. Membranes with calcium phosphates had better osteoblast attachment, proliferation and migration. Taken together, results indicate the great potential of PLGA/HAp/β-TCP bilayer membranes on bone reconstruction with proper degradation profile, morphology, mechanical behavior and bone regenerative capacity.
引导骨再生膜是一种有价值的资源，可防止成纤维细胞浸润，并有助于解剖骨重建。然而，现有的膜缺乏骨再生能力、合适的机械性能或适当的降解特性。因此，为了克服这些限制，本研究开发了双层膜，其致密层（干燥相转化）为 PLGA（聚乳酸-共-羟基乙酸）：HAp（羟基磷灰石）-95:05（wt%），电纺层为 PLGA 和 HAp：β-TCP（β-磷酸三钙），比例为 60:40、70:30 和 85:15（wt%），评估其机械、形态和体外性能。双层膜具有适当的层间附着力，致密层的孔径为 4.20μm，电纺层的孔隙度为 38.2%，因此既能防止成纤维细胞浸润，又能允许成骨细胞迁移和营养渗透。它们还表现出 82°C 的 T 和更高的储能模量，在 54.6°C 以下保持恒定，这些特性对于膜的植入和使用非常重要，没有机械上的妥协。在体外降解 60 天后，质量损失仅为 10%，这种降解特性适合应用要求。含有钙磷的膜具有更好的成骨细胞附着、增殖和迁移能力。综上所述，结果表明 PLGA/HAp/β-TCP 双层膜具有适当的降解特性、形态、机械性能和骨再生能力，在骨重建方面具有巨大的潜力。
