Li Junxuan, Ding Jiaxin, Zhou Tao, Li Bolun, Wang Jingjing, Wang Hanchi, Fu Li
Department of Oral Implantology, Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Hospital of Stomatology, Jilin University, Changchun, China.
Front Pharmacol. 2024 Sep 27;15:1453036. doi: 10.3389/fphar.2024.1453036. eCollection 2024.
Guided bone regeneration (GBR) technology has been widely used as a reliable method to address alveolar bone defects. To improve the clinical effects of GBR approach, there have been attempts to develop barrier membranes with enhanced regenerative properties. However, modifying the material and structure of GBR membranes to integrate physicochemical properties and biological activity remains challenging. The aim of this study was to develop a novel functionally graded bilayer membrane (FGBM) with a gradient structure and composition, and to evaluate its osteogenesis promotion effect for GBR.
By combining the phase inversion method and electrospinning method, functionally graded bilayer membranes (FGBM) with gradient structure and composition of poly(lactic-co-glycolic acid) (PLGA), nano-hydroxyapatite (nHA), and gelatin were fabricated in this study. The physicochemical and biological properties of the prepared FGBM, including structural and morphological characterization, mechanical properties, biodegradation, cell behaviors, and osteogenic bioactivity, were comprehensively evaluated.
The findings demonstrated the successful fabrication of PLGA/nHA/gelatin FGBM with an asymmetric structure, exhibiting enhanced hydrophilic, mechanical, and degradation properties. The incorporation of gelatin not only improved the biological integration, but also enhanced the binding affinity between electrospun fiber layer and phase inversion layer. The FGBM with a 30% nHA mass fraction and a PLGA/gelatin mass ratio of 1:1 exhibited excellent barrier function and osteogenic bioactivities and .
This work demonstrated the potential of PLGA/nHA/gelatin FGBM in bone regeneration and provided valuable insight for the development of barrier membrane.
引导骨再生（GBR）技术已被广泛用作解决牙槽骨缺损的可靠方法。为了提高GBR方法的临床效果，人们一直在尝试开发具有增强再生性能的屏障膜。然而，改变GBR膜的材料和结构以整合物理化学性质和生物活性仍然具有挑战性。本研究的目的是开发一种具有梯度结构和组成的新型功能梯度双层膜（FGBM），并评估其对GBR的成骨促进作用。
本研究通过结合相转化法和静电纺丝法，制备了具有聚乳酸-乙醇酸共聚物（PLGA）、纳米羟基磷灰石（nHA）和明胶梯度结构和组成的功能梯度双层膜（FGBM）。对制备的FGBM的物理化学和生物学性质，包括结构和形态表征、力学性能、生物降解、细胞行为和成骨生物活性进行了全面评估。
研究结果表明成功制备了具有不对称结构的PLGA/nHA/明胶FGBM，其具有增强的亲水性、力学性能和降解性能。明胶的加入不仅改善了生物整合性，还增强了静电纺丝纤维层与相转化层之间的结合亲和力。nHA质量分数为30%且PLGA/明胶质量比为1:1的FGBM表现出优异的屏障功能和成骨生物活性。
这项工作证明了PLGA/nHA/明胶FGBM在骨再生中的潜力，并为屏障膜的开发提供了有价值的见解。
